Abstract
Bats possess a genome approximately 50–87% the size of other eutherian mammals. We document that the events that have achieved or maintained a small genome size in the Mexican leaf-nosed bat Macrotus waterhousii have resulted in a lower copy number of interspersed and tandemly repetitive elements. These conclusions are based on examination of 1726 randomly chosen recombinant cosmids, with an average insert size of 35.7 kb and representing 2.6% of the haploid genome of M. waterhousii. Probes representative of microsatellites [(GT)n, (CT)n, (AT)n, (GC)n] and a tandem repeat (rDNA) were used to estimate frequency of repetitive elements in the M. waterhousii genome. Of the four dinucleotides, (GT)n was present in 33.5% of the clones, (CT)n was present in 31.0% of the clones, and (AT)n and (GC)n were not represented in any of the clones examined. The 28S rDNA and a repetitive element from M. californicus were found in three and four clones, respectively. The dinucleotides (GT)n and (CT)n occurred together in the same clone more frequently than expected from chance. Although our data do not allow us to empirically test which mechanisms are maintaining copy number of repetitive DNA in the bat genome, the nonrandom association of these different families of repetitive DNA may provide insight into a mechanism that proportionately reduces diverse families of repetitive DNA that are known to be amplified by very different mechanisms.
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References
Arnheim, N. (1979). Characterization of mouse ribosomal genome fragments purified by molecular cloning. Gene 7, 83–96.
Bachmann, K. (1972). Genome size in mammals. Chromosoma 37, 85–93.
Baker, R.J., Honeycutt, R.L., Van Den Bussche, R.A. (1991). Examination of monophyly of bats: restriction map of the ribosomal DNA cistron. In Contributions to Mammalogy in Honor of Karl F. Koopman, T.A. Griffiths, D. Klingener, eds. pp. 42–53. Bull. Am. Mus. Nat. Hist. 206, 1–432.
Baker, R.J., Maltbie, M., Owen, J.G., Hamilton, M.J., Bradley, R.D. (1992). Reduced number of ribosomal sites in bats: evidence for a mechanism to contain genome size. J. Mammal. 73, 847–858.
Baker, R.J., Longmire, J.L., Van Den Bussche, R.A. (1995). Organization of repetitive elements in the Upland cotton genome (Gossypium hirsutum). J. Hered. 86, 178–185.
Beckman, J.S., Weber, J.L. (1992). Survey of human and rat microsatellites. Genomics 12, 627–631.
Burton, D. W., Bickham, J. W., Genoways, H. H. (1989). Flow-cytometric analyses of nuclear DNA content in four families of Neotropical bats. Evolution 43, 756–765.
Capanna, E., Manfredi-Romanini, M.G. (1971). Nuclear DNA content and morphology of the karyotype in certain Palearctic Microchiroptera. Caryologia 24, 471–481.
Cavalier-Smith, T. (1985). Introduction: the evolutionary significance of genome size. In The Evolution of Genome Size, T. Cavalier-Smith, ed. (New York: John Wiley & Sons), pp. 1–36.
Crampton, J.M., Davies, K.E., Knapp, T.P. (1981). The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes. Nucleic Acids Res. 9, 3821–3834.
Evans, G.A., Lewis, K., Rothberg, B.E. (1989). High efficiency vectors for cosmid microcloning and genomic analysis. Gene 79, 9–20.
Flavell, R.B., Bennett, M.D., Smith, J.B., Smith D.B. (1974). Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12, 257–269.
Hamada, H., Petrino, M.G., Kakunaga, T. (1982). A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc. Natl. Acad. Sci. USA 79, 6465–6469.
Hamilton, M.J., Honeycutt, R.L., Baker, R.J. (1990). Intragenomic movement, sequence amplification and concerted evolution in satellite DNA in harvest mice, Reithrodontomys: evidence from in situ hybridization. Chromosoma 99, 321–329.
Hamilton, M.J., Hong, G., Wichman, H.A. (1992). Intragenomic movement and concerted evolution of satellite DNA in Peromyscus: evidence from in situ hybridization. Cytogenet. Cell Genet. 60, 40–44.
Hinegardner, R. (1976). Evolution of genome size. In Molecular Evolution, F.J. Ayala, ed. (Sunderland, Mass.: Sinauer Associates, Inc.), pp. 179–199.
Janecek, L.L., Longmire, J.L., Wichman, H.A., Baker, R.J. (1993). Organization of repetitive elements in the genome of the white-footed mouse, Peromyscus leucopus. Mamm. Genome 4, 374–381.
Kato, H., Harada, M., Tsuchiya, K., Moriwaki, K. (1980). Absences of correlation between DNA repair in ultraviolet irradiated mammalian cells and lifespan of the donor species. Jpn. J. Genet. 55, 99–108.
Lapitan, N.L.V. (1993). Organization and evolution of higher plant nuclear genomes. Genome 35, 171–181.
Longmire, J.L., Ambrose, R.E., Brown, N.C., Cade, T.J., Maechtle, T.L., Seeger, W.S., Ward, F.P., White, C.M. (1991). Use of sex-linked minisatellite fragments to investigate genetic differentiation and migration of North American populations of the Peregrine Falcon (Falco peregrinus). In DNA Fingerprinting: Approaches and Applications, T. Burke, G. Dolf, A. Jeffreys, R. Wolff, eds. Brasil: Birkhauser Press), pp. 217–229.
Longmire, J.L., Brown, N.C., Meoncke, Campbell, M.L., Albright, K.L., Fawcett, J.J., Campbell, E.W., Moyzis, R.K., Hildebrand, C.E., Evans, G.A., Deaven, L.L. (1993). Construction and characterization of partial digest libraries made from flow sorted human chromosome 16. Genet. Anal. Tech. Appl. 10, 69–76.
Moyzis, R.K., Torney, D.C., Meyne, J., Buckingham, J.M., Wu, J.-R., Burk, C., Sirotkin, K.M., Goad, W.B. (1989). The distribution of interspersed repetitive DNA sequences in the human genome. Genomics 4, 273–289.
Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Plainview, N.Y.: Cold Spring Harbor Laboratory Press).
Shaeffer, R.L., Mendenhall, W., Ott, L. (1990). Elementary Survey Sampling, 4th ed. (Belmont, Calif.: Duxbury Press).
Stallings, R.L. (1992). CpG suppression in vertebrate genomes does not account for the rarity of (CpG)n microsatellite repeats. Genomics 17, 1520–1521.
Stallings, R.L., Ford, A.F., Nelson, D., Torney, D.C., Hildebrand, C.E., Moyzis, R. (1991). Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics 10, 807–815.
Swift, H. (1950). The constancy of deoxyribose nucleic acid in plant nuclei. Proc. Natl. Acad. Sci. USA 36, 643–654.
Szarski, H. (1974). Cell size and nuclear DNA content in vertebrates. Int. Rev. Cytol. 23, 459–467.
Tiersch, T.R., Wachtel, S.S. (1991). On the evolution of genome size in birds. J. Hered. 82, 363–368.
Van Den Bussche, R.A. (1991). Phylogenetic analysis of restriction site variation in the ribosomal DNA complex of the New World leaf-nosed bat genera. Syst. Zool. 40, 420–432.
Van Den Bussche, R.A. (1992). Restriction-site variation and molecular systematics of new World leaf-nosed bats. J. Mammal. 73:29–42.
Wu, C-I., Hammer, M.F. (1991). Molecular evolution of ultraselfish genes of meiotic drive systems. In Evolution at the Molecular Level, R.K. Selander, A.G. Clark, T.S. Whittam, eds. (Sunderland, Mass.: Sinauer Associates, Inc.), pp. 177–203.
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Van Den Bussche, R.A., Longmire, J.L. & Baker, R.J. How bats achieve a small C-value: frequency of repetitive DNA in Macrotus . Mammalian Genome 6, 521–525 (1995). https://doi.org/10.1007/BF00356168
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DOI: https://doi.org/10.1007/BF00356168