Summary
Ten dominant omnipotent suppressors of Saccharomyces cerevisiae, which were previously shown to be different from SUP46, have been examined. Nine are mapped in a region between lys5 and cyh2 on the left arm of chromosome VII. These suppressors, like SUP46, manifest sensitivity to increased temperature and the antibiotics paromomycin and hygromycin B. In addition, they have an identical action spectrum. These results strongly suggest that they are allelic to each other and they are designated SUP138. The tenth is mapped to a position between his1 and arg6 on the right arm of chromosome V. This suppressor, named SUP139, does not manifest temperature sensitivity nor antibiotic sensitivity. SUP139 and SUP138, which are clearly distinguished by means of action spectrum, act on much fewer nonsense mutations than SUP46. It is now clear that dominant omnipotent suppressors arising at a single locus are homogeneous and that their efficiency is locus-dependent. The order of efficiency is SUP46>SUP138>SUP139.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breining P, Piepersberg W (1986) Nucleic Acids Res 14:5187–5197
Chattoo BB, Palmer B, Ono B, Sherman F (1979) Genetics 93:67–79
Cottrelle P, Thiele D, Price VL, Memet S, Micouin J-Y, Marck C, Buhler J-M, Stenac A, Fromageot P (1985) J Biol Chem 260:3090–3096
Cox BS (1977) Genet Res 30:187–205
Crouzet M, Izgu F, Grant CM, Tuite MF (1988) Curr Genet 14:537–543
Crouzet M, Tuite MF (1989) Mol Gen Genet 210:591–583
Culbertson MR, Gaber RF, Cummins CM (1982) Genetics 102:745–764
Eustice DC, Waken LP, Wilhelm JM, Sherman F (1986) J Mol Biol 188:207–214
Gélugne J-P, Bell JB (1988) Curr Genet 14:345–354
Gerlach WL (1975) Mol Gen Genet 138:53–63
Guthrie C, Abelson J (1982) Organization and expression of tRNA genes in Saccharomyces cerevisiae. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 487–528
Hawthorne DC, Leupold U (1974) Curr Topics Microbiol Immuno 64:1–47
Himmelfarb HJ, Maicas E, Friesen JD (1985) Mol Cell Biol 5:816–822
Ishiguro J, Ono B, Masurekar M, McLaughlin CS, Sherman F (1981) J Mol Biol 147:391–397
Kikuchi Y, Shimatake H, Kikuchi A (1988) EMBO J 7:1175–1182
Kushinirov VV, Ter-Avenesyan MD, Surguchov AP, Smirnov VN, Inge-Vechtomov SG (1987) FEBS Lett 215:257–260
Kushinirov VV, Ter-Avenesyan MD, Telckov MV, Surguchov AP, Smirnov VN, Inge-Vechtomov SG (1988) Gene 66:45–54
Masurekar M, Palmer E, Ono B, Wilhelm JM, Sherman F (1981) J Mol Biol 147:381–390
Matsumoto K, Adachi A, Toh-e A, Oshima Y (1980) J Bacteriol 14:508–521
McCusker JH and Haber JE (1988a) Genetics 119:303–315
McCusker JH and Haber Je (1988b) Genetics 119:316–327
Mortimer RK, Hawthorne DC (1975) Genetic mapping in yeast. In: Prescott DM (ed) Methods in cell biology, vol 11, Academic Press, New York, pp 221–233
Mortimer RK, Shild D (1980) Microbiol Rev 44:519–571
Nagata S, Nagashima K, Tsunetsugi-Yokota Y, Fujimura K, Miyazaki M, Kaziro (1984) EMBO J 3:1825–1830
Ono B, Ishino-Arao Y, Shirai T, Maeda N, Shinoda S (1985) Curr Genet 9:197–203
Ono B, Ishino-Arao Y, Tanaka M, Awano I, Shinoda S, (1986) Genetics 114:363–374
Ono B, Moriga N, Ishihara K, Ishiguro J, Ishino Y, Shinoda S (1984) Genetics 107:219–230
Ono B, Stewart JW, Sherman F (1981a) J Mol Biol 147:373–379
Ono B, Wills N, Stewart JW, Gestland RF, Sherman F (1981b) J Mol Biol 150:361–373
Papciak SM, Pearson NJ (1987) Curr Genet 11:445–450
Perkins TD (1949) Genetics 34:607–626
Sandbaken MG, Culbertson MR (1988) Genetics 120:923–934
Schirmaier F, Philippsen P (1984) EMBO J 3:3311–3315
Sherman F (1982) Suppression in the yeast Saccharomyces cerevisiae. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 463–486
Sherman F, Fink G, Lawrence C (1974) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Harbor, New York
Sherman F, Ono B, Stewart JW (1979) Use of iso-1-cytochrome c system for investigating nonsense mutants and suppressors in yeast. In: Celis JE, Smith JD (eds) Nonsense mutations and tRNA suppressors Academic Press, London, pp 133–153
Skogerson L, Wakatama E (1976) Proc Natl Acad Sci USA 73:73–76
Smirnov VN, Krier VG, Lizlova LV, Andrianova VM, Inge-Vechtomov SG (1974) Mol Gen Genet 129:105–121
Smirnov VN, Surguchov AP, Smirnov VV, Beresteskaya YuV, Inge-Vechtomov SG (1978) Mol Gen Genet 163:87–90
Song JM, Liebman SW (1987) Genetics 115:451–460
Stocklein W, Pipersberg W (1980) Curr Genet 1:177–183
Surguchov AP, Beresteskaya YuV, Fominykch ES, Pospelova EM, Smirnov VN, Ter-Avenesyan MD, Inge-Vechtomov SG (1980) FEBS Lett 111:175–178
Wilson PG, Culbertson MR (1988) NATO ASI Ser/Ser H 14:415–430
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ono, Bi., Tanaka, M., Awano, I. et al. Two new loci that give rise to dominant omnipotent suppressors in Saccharomyces cerevisiae . Curr Genet 16, 323–330 (1989). https://doi.org/10.1007/BF00340710
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00340710