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Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli

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Summary

The host-controlled EcoK-restriction of unmodified phage λ.O is alleviated in dam mutants of Escherichia coli by 100- to 300-fold. In addition, the EcoK modification activity is substantially decreased in dam - strains. We show that type I restriction (EcoB, EcoD and EcoK) is detectably alleviated in dam mutants. However, no relief of EcoRI restriction (Type II) occurs in dam - strains and only a slight effect of dam mutation on EcoP1 restriction (Type III) is observed. We interpret the alleviation of the type I restriction in dam - strains to be a consequence of induction of the function which interferes with type I restriction systems.

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Authors and Affiliations

  1. Institute of Experimental Cardiology, National Cardiology Research Center, USSR Academy of Medical Sciences, 121552, Moscow, USSR

    E. P. Efimova, E. P. Delver & A. A. Belogurov

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  1. E. P. Efimova
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  2. E. P. Delver
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  3. A. A. Belogurov
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Communicated by W. Arber

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Efimova, E.P., Delver, E.P. & Belogurov, A.A. Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli . Mol Gen Genet 214, 313–316 (1988). https://doi.org/10.1007/BF00337727

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  • DOI: https://doi.org/10.1007/BF00337727

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