Summary
A DNA fragment called suc1 has been found to rescue cells mutated in the cell cycle control gene cdc2 of the fission yeast Schizosaccharomyces pombe. The suppressing activity of suc1 is observed when it is present on a multicopy number plasmid. The gene does not hybridise to cdc2 and maps elsewhere in the genome. Its effect is cdc2 allele specific suggesting that it interacts directly with the cdc2 gene function.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beach D, Durkacz B, Nurse P (1982) Functionally homologous cell cycle control genes in budding and fission yeast. Nature 300:706–709
Beach D, Piper M, Nurse P (1982) Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation. Mol Gen Genet 187:326–329
Durkacz B, Beach D, Hayles J, Nurse P (1985) The fission yeast cell cycle control gene cdc2: structure of the cdc2 region. Mol Gene Genet 201:543–545
Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RC (ed) Handbook of Genetics, vol 1, Plenum Press, New York, pp 295–346
Hindley J, Phear G (1984) Sequence of the cell division gene cdc2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases. Gene 31:129–134
Lorincz A, Reed S (1984) Primary structure homology between the product of yeast cell division control gene cdc28 and vertebrate oncogeneses. Nature 307:183–185
Nurse P (1985) Cell cycle control genes in yeast. Trends Genet 1:51–55
Nurse P, Bissett Y (1981) Cell cycle gene required in G1 for commitment to cell division and in G2 for control of mitosis in fission yeast. Nature 292:558–560
Nurse P, Thuriaux P (1980) Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe. Genetics 96:627–637
Pringle J, Hartwell L (1981) The Saccharomyces cerevisiae cell cycle. In: Strathern J, Jones E, Broach J (eds) The Molecular Biology of the Yeast Saccharomyces. Cold Spring Harbor Laboratory, New York, pp 97–143
Author information
Authors and Affiliations
Additional information
Communicated by C. Auerbach
Rights and permissions
About this article
Cite this article
Hayles, J., Beach, D., Durkacz, B. et al. The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function. Mol Gen Genet 202, 291–293 (1986). https://doi.org/10.1007/BF00331653
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331653