Abstract
Among 35 Rhizobium isolates of Acacia nilotica, from different agro-climatic zones, two, ANG4 and ANG5, tolerated up to 850 mm NaCl and one, ANG3, was sensitive to NaCl above 250 mm. Nodulation and nitrogenase activity of the three isolates decreased with increasing concentration of salt up to 150 mm. Nodulation by ANG3 was 15% at 75 mm NaCl and nil at 100 mm. With ANG4 and ANG5, nodulation was only slightly decreased at 150 mm NaCl. Nitrogenase activity associated with plants inoculated with ANG3 was halved at 25 mm NaCl compared with salt-free controls, whereas isolates ANG4 and ANG5 retained 25% and 15% activity, respectively, even at 100 mm NaCl. Salt-tolerant Rhizobium isolates can therefore nodulate and fix N2 in saline soils.
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References
Craig, G.F., Atkins, C.A. & Bell, D.T. 1991 Effect of salinity on growth of four strains of Rhizobium and their infectivity and effectiveness on two species of Acacia. Plant and Soil 133, 253–262.
Hafeez, F.Y., Aslam, Z. & Malik, K.A. 1988 Effect of salinity and inoculation on growth, nitrogen fixation and nutrient uptake of Vigna radiata (L) Wilczek. Plant and Soil 106, 3–8.
Hua, S.T., Tsai, V.Y., Lichens, G.M. & Noma, A.T. 1982 Accumulation of amino acids in Rhizobium sp. strain WR 110 in response to sodium chloride salinity. Applied and Environmental Microbiology 44, 135–140.
Kassem, M., Capellano, A. & Gounot, A.M. 1985 Effects du chlorure de sodium sur la croissance in vitro, l'infectivite et l'efficience de Rhizobium meliloti. Mircen Journal of Applied Microbiology and Biotechnology 1, 63–75.
Krieg, N.R. & Holt, J.G. 1984 Bergey's Manual of Systematic Bacteriology, Vol 1. Baltimore: Williams & Wilkins.
Lauter, D.J., Munns, D.N. & Clarkin, K.L. 1981 Salt response of chickpea as influenced by nitrogen supply. Agronomy Journal 73, 961–966.
Singleton, P.W. & Bohlool, B.B. 1984 Effect of salinity on nodule formation by soybean. Plant Physiology 74, 72–76.
Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook No. 15. Edinburgh: Blackwell.
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Lal, B., Khanna, S. Selection of salt-tolerant Rhizobium isolates of Acacia nilotica . World J Microbiol Biotechnol 10, 637–639 (1994). https://doi.org/10.1007/BF00327949
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DOI: https://doi.org/10.1007/BF00327949