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High-level resistance to cycloheximide resulting from an interaction of the mutated pdr3 and cyh genes in yeast

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Summary

In addition to pdr3-1, the S. cerevisiae nuclear pleiotropic drug resistance mutant 2D was found to contain another recessive nuclear mutation, cyh, conferring specific resistance to cycloheximide only. The cycloheximide resistance level due to either the pdr3-1 or the cyh mutation alone was low and was not altered by the ogd1 mutation which increased the physiological acidification of the culture. When pdr3-1 and cyh mutations occurred simultaneously in the haploid yeast strain their interaction was synergistic and resulted in high-level resistance to cycloheximide.

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Authors and Affiliations

  1. Department of Microbiology and Virology, Comenius University, Mlynská dolina B-2, 842 15, Bratislava, Czechoslovakia

    Branislav Ruttkay-Nedecký, Margita Obernauerová & Július Šubík

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  1. Branislav Ruttkay-Nedecký
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  2. Margita Obernauerová
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  3. Július Šubík
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Ruttkay-Nedecký, B., Obernauerová, M. & Šubík, J. High-level resistance to cycloheximide resulting from an interaction of the mutated pdr3 and cyh genes in yeast. Curr Genet 22, 337–339 (1992). https://doi.org/10.1007/BF00317932

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  • DOI: https://doi.org/10.1007/BF00317932

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