Abstract
Cell type in the yeast Saccharomyces cerevisiae is determined by information present at the MAT locus. Cells can switch mating types when cell-type information located at a silent locus, HML or HMR, is transposed to the MAT locus. The HML and HMR loci are kept silent through the action of a number of proteins, one of which is the DNA-binding protein, ABF1. We have identified a binding site for ABF1 within the Ya region of MAT a and HMR a. In order to examine the function of this ABF1-binding site, we have constructed strains that lack the site in the MAT a or HMR a loci. Consistent with the idea that ABF1 plays a redundant role in silencing, it was found that a triple deletion of the ABF1-binding sites at HMRE, Ya and I did not permit the expresion of HMR a. We have also shown that chromosomal deletion of the binding site at MATY a had no effect on the level of cutting by the HO endonuclease nor on the amount of mating-type switching observed. Similarly, chromosomal deletion of all three ABF1-binding sites at HMR a had no effect on the directionality of mating-type switching.
Similar content being viewed by others
References
Abraham J, Nysmyth KA, Stratern JN, Klar AJS, Hicks JB (1994) J Mol Biol 176:307–331
Aparicio OM, Billington BL, Gottschling DE (1991) Cell 66:1279–1287
Astell CR, Ahlstrom-Jonasson L, Smith M, Tatchell K, Nasmyth KA, Hall BD (1981) Cell 27:15–23
Bell SP, Kobayashi R, Stillman B (1993) Science 262:1844–1849
Boeke JD, LaCroute F, Fink GR (1984) Mol Gen Genet 197:345–346
Brand AH, Micklem G, Nasmyth K (1987) Cell 51:709–719
Buchman AR, Kornberg RD (1990) Mol Cell Biol 10:887–897
Buchman A, Kimmerly WJ, Rine J, Kornberg RD (1988) Mol Cell Biol 8:210–225
Chan RK, Otte CA (1982) Mol Cell Biol 2:11–20
Chien C, Buck S, Sternglanz R, Shore D (1993) Cell 75:531–541
Connolly B, White C, Haber JE (1988) Mol Cell Biol 8:2342–2349
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Feldman JB, Hicks JB, Broach JR (1984) J Mol Biol 178:815–834
Foss M, McNally F, Laurenson P, Rine J (1993) Science 262: 1838–1844
Hardy CFJ, Sussel L, Shore D (1992) Genes Dev 6:801–814
Hofmann JF-X, Laroche T, Brand AH, Gasser SM (1989) Cell 57:725–737
Jensen RE, Herskowitz I (1984) Cold Spring Harbor Symp Quant Biol 49:97–104
Jensen R, Sprague GF, Herskowitz I (1983) Proc Natl Acad Sci USA 80:3035–3039
John B (1988) The biology of heterochromatin. In: Verma RS (ed) Heterochromatin, Cambridge University Press, Cambridge, pp 1–147
Kayne PS, Kim UJ, Han M, Mullen JR, Yoshizaki F, Grunstein M (1988) Cell 55:27–39
Kimmerly W, Buchman A, Kornberg R, Rine J (1988) EMBO J 7:2241–2253
Klar AJS, McIndoo J, Strathern JN, Hicks JB (1980) Cell 22:291–298
Klar AJS, Hicks JB, Strathern JN (1982) Cell 28:551–561
Klar AJS, Strathern JN, Hicks JB, Prudente D (1983) Mol Cell Biol 3:803–810
Kurtz S, Shore D (1991) Genes Dev 5:616–628
Lee D, Sadowski PD (1981) J Virol 40:839–847
Mahoney DJ, Marquardt R, Shei G-J, Rose AB, Broach JR (1991) Genes Dev 5:605–615
Marahrens Y, Stillman B (1992) Science 255:817–823
Megee PC, Morgan BA, Mittman BA, Smith MM (1990) Science 247:841–845
McBroom LDB (1993) PhD thesis, University of Toronto
McBroom LDB, Sadowski PD (1994) J Biol Chem 269:16455–16460
Micklem G, Rowley A, Harwood J, Nasmyth K, Diffley JFX (1993) Nature 366:87–89
Mullen JR, Kayne PS, Moerschell RP, Tsunasawa M, Gribskov M, Colavito-Shepanski M, Grunstein M, Sherman F, Sternglanz R (1989) EMBO J 8:2067–2075
Nakamaye KL, Eckstein F (1986) Nucleic Acids Res 14:9679–9698
Nasmyth KA (1982) Cell 30:567–578
Palladino F, Laroche T, Gilson E, Axerod A, Pillush L, Gasser SM (1993) Cell 75:543–555
Petersen Bjørn S, Soltyk A, Beggs JD, Friesen JD (1989) Mol Cell Biol 9:3698–3709
Pillus L, Rine J (1989) Cell 59:637–647
Proteau G, Sidenberg D, Sadowski P (1986) Nucleic Acids Res 14:4787–4802
Rhode PR, Elsasser S, Campbell UL (1992) Mol Cell Biol 12: 1064–1077
Rine J, Herskowitz I (1987) Genetics 116:9–22
Rose M, Grisafi P, Botstein D (1984) Gene 29:113–124
Russell DW, Jensen R, Zoller MJ, Burke J, Errede B, Smith M, Herskowitz I (1986) Mol Cell Biol 6:4281–4294
Sambrook J, Fritsch EF, Maniatis T (1989) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sherman F, Fink GR, Hicks JB (1986) Cold Spring Harbour Laboratory. Cold Spring Harbor, New York
Shore D, Nasmyth K (1987) Cell 51:721–732
Shore D, Stillman DJ, Brand AH, Nasmyth KA (1987) EMBO J 6:461–467
Sikorski RS, Hieter P (1989) Genetics 122:19–27
Spradling AC, Karpen GH (1990) Genetics 126:779–784
Sprague GF (1991) Methods Enzymol 194:77–93
Stern M, Jensen R, Herskowitz I (1984) J Mol Biol 178:853–868
Stone EM, Swanson MJ, Romeo AM, Hicks JB, Sternglanz R (1991) Mol Cell Biol 11:2253–2262
Strathern J, Hicks J, Herskowitz I (1981) J Mol Biol 147:357–372
Strathern JN, Klar AJS, Hicks JB, Abraham JA, Ivy JM, Nasmyth KA, McGill C (1982) Cell 31:183–192
Trawick JD, Kraut N, Simon FR, Poyton RO (1992) Mol Cell Biol 12:2302–2314
Walker SS, Francesconi SC, Tye BK, Esisenberg S (1989) Mol Cell Biol 9:2914–2921
Weiler KS, Broach JR (1992) Genetics 132:929–942
White CI, Haber JE (1990) EMBO J 9:663–673
Whiteway M, Freeman R, Van Arsdell S, Szostak JW, Thorner J (1987) Mol Cell Biol 7:3713–3722
Zwieb C, Kim J, Adhya S (1989) Genes Dev 3:606–611
Author information
Authors and Affiliations
Additional information
Communicated by R. J. Rothstein
Rights and permissions
About this article
Cite this article
McBroom, L.D.B., Sadowski, P.D. Functional analysis of the ABF1-binding sites within the Ya regions of the MATa and HMRa loci of Saccharomyces cerevisiae . Curr Genet 28, 1–11 (1995). https://doi.org/10.1007/BF00311875
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00311875