Abstract
In interphase cells of Aedes aegypti (L.) (2n=4+ XX/XY), only the nucleolus responded to selective silver staining. The secondary constriction on chromosome 3 remained unresponsive at all times but the six centromeres were identified throughout mitosis from early prophase as well as those stages of meiosis subsequent to diplotene. The centromeric blocks were not synonymous with the pericentric heterochromatin revealed by C-banding. X chromosomes without an intercalary C-band were newly discovered in Ae. aegypti in the Bangalore strain. Sequential Q-or Hoechst 33258/C-banding in this and the Trinidad-30 strain revealed intercalary heterochromatin diversity within and between strains and also differences between intercalary and pericentric heterochromatin.
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References
Angelier N, Hernandez-Verdun D, Bouteille M (1982) Visualisation of Ag-NOR proteins on nucleolar transcriptional units in molecular spreads. Chromosoma 86:661–672
Buys HCM, Osinga J (1980) Abundance of protein bound sulphydryl and disulphide groups at chromosomal nucleolus organizing regions. Chromosoma 77:1–11
Comings DE, Drets ME (1976) Mechanisms of chromosome banding IX. Are variations in DNA base composition adequate to account for quinacrine, Hoechst 33258 and daunamycin banding? Chromosoma 56:199–211
Goodpasture C, Bloom SE (1975) Visualisation of nucleolar organizing regions in mammalian chromosomes using silver staining. Chromosoma 53:37–50
Marchi A, Rai KS (1986) Chromosome banding homologies in three species of Aedes (Stegomyia). Can J Genet Cytol 28:198–202
Mayr B, Rab P, Kalat M (1986) Localisation of NORs and counterstain enhanced fluorescence studies in Salmo gairdneri and Salmo trutta (Pisces, Salmonidae). Theor Appl Genet 71:703–707
Motara MA, Rai KS (1977) Chromosomal differentiation in two species of Aedes and their hybrids revealed by Giemsa C-banding. Chromosoma 64:125–132
Motara MA, Rai KS (1978) Giemsa C-banding patterns in Aedes (Stegomyia) mosquitoes. Chromosoma 64:125–132
Motara MA, Pathak S, Satya-Prakash KL, Hsu TC (1985) Argentophilic structures of spermatogenesis in the yellow fever mosquito. J Hered 76:295–300
Müller W, Gautier E (1975) Interactions of heterochromatic compounds with nucleic acids. AT-specific non-intercalating DNA ligands. Eur J Biochem 54:385–394
Newton ME (1985) Heterochromatin diversity in two species of Pellia (Hepaticae) as revealed by C-, Q-, N-and Hoechst 33258-banding. Chromosoma 92:1–9
Newton ME, Southern DI, Wood RJ (1974) X and Y chromosomes of Aedes aegypti (L.) distinguished by Giemsa C-banding. Chromosoma 49:41–49
Newton ME, Wood RJ, Southern DI (1976) A cytogenetic analysis of meiotic drive in the mosquito Aedes aegypti (L.). Genetica 46:297–318
Newton ME, Southern DI, Wood RJ (1978a) Relative DNA content of normal and sex ratio distorting spermatazoa of the mosquito Aedes aegypti (L.). Chromosoma 67:253–261
Newton ME, Wood RJ, Southern DI (1978b) Cytological mapping of the M and D loci in the mosquito Aedes aegypti (L.). Genetica 48:137–143
Pachmann U, Rigler R (1972) Quantum yield of acridines interacting with DNA of defined base sequence. A basis for the explanation of acridine bands in chromosomes. Exp Cell Res 72:602–608
Schwarzacher HG, Mikelsaar AV, Schnedl W (1978) The nature of the Ag-staining of nucleolus organising regions. Cytogenet Cell Genet 20:24–39
Sentis C, Santos J, Fernandez-Piqueras J (1986) C-heterochromatin polymorphism in Baetica ustulata: Intraindividual variation and fluorescence banding patterns. Chromosoma 94:65–70
Shi L, Pathak S, Hsu TC (1982) Demonstration of kinetochores and centrioles in spermatocytes of two species of cockroaches by silver staining. Chromosoma 85:421–426
Suguna SG, Wood RJ, Curtis CF, Whitelaw A, Kazmi SJ (1977) Resistance to meiotic drive at the MD locus in an Indian wild population of Aedes aegypti (L.). Genet Res 29:123–132
Sumner AT (1982) The nature and mechanisms of chromosome banding. Cancer Genet Cytogenet 6:59–87
Wood RJ (1976) Between-family variation in sex ratio in the Trinidad (T-30) strain of Aedes aegypti (L.) indicating differences in sensitivity to the meiotic drive gene MD. Genetica 46:345–361
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Wallace, A.J., Newton, M.E. Heterochromatin diversity and cyclic responses to selective silver staining in Aedes aegypti (L.). Chromosoma 95, 89–93 (1987). https://doi.org/10.1007/BF00293847
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DOI: https://doi.org/10.1007/BF00293847