Abstract
One hundred and one new dinucleotide repeat polymorphisms specific for the canine genome have been identified and characterized. Screening of both primary libraries and marker selected libraries enriched for simple sequence repeats led to the isolation of large numbers of genomic clones that contained (CA)n repeats. Over 200 of these clones were sequenced, and PCR primers that bracket the repeat were developed for those that contained ten or more continuous (CA)n units. This effort led to the production of 101 polymorphic markers, which were assigned to one of four categories depending on their degree of polymorphism. Fiftyfour markers were found to be highly or very highly polymorphic as they had four or more alleles when tested on a panel of unrelated dogs. This group of markers will be useful for following inheritance of traits in crosses between dogs.
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Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., et al. (1994). A genetic linkage map of the bovine genome. Nature Genet. 6, 227–234.
Dietrich, W., Katz, H., Lincoln, S.E., Shin, H.-S., Friedman, J., Dracopoli, N.C., Lander, E.S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447.
Gottelli, D., Sillero-Zubiri, C., Applebaum, G.D., Roy, M.S., Girman, D.J., Garcia-Moreno, J., Ostrander, E.A., Wayne, R.K. (1994). Molecular genetics of the most endangered canid: the Ethiopian wolf, Canis simensis. Mol. Ecol. 3, 301–312.
Hamada, H., Kakunaga, T. (1982). Potential Z-forming sequences are highly dispersed in the human genome. Nature 298, 396–398.
Hamada, H., Petrino, M.G., Kakunaga, T., Seidman, M., Stollar, B.D. (1984). Characterization of genomic Poly(dT-dG)·Poly(dC-dA) sequences; structure, organization and conformation. Mol. Cell. Biol. 4, 2610–2621.
Hearne, C.M., McAleer, M.A., Love, J.M., Aitman, T.J., Cornall, R.J., Ghosh, S., Knight, A.M., Prins, J.B., Todd, J.A. (1991). Additional microsatellite markers for mouse genome mapping. Mamm. Genome 1, 273–282.
Litt, M., Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet. 44, 397–401.
Miesfeld, R., Krystal, M., Arnheim, N. (1981). A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human delta and beta globin genes. Nucleic Acids Res. 9, 5931–5947.
Moore, S.S., Sargeant, L.L., King, T.J., Mattick, J.S., Georges, M., Hetzel, D.J. (1991). The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics 10, 654–660.
Ostrander, E.A., Jong, P.M., Rine, J., Duyk, G. (1992). Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences. Proc. Natl. Acad. Sci. USA 89, 3419–3423.
Ostrander, E.A., Sprague, G., Rine, R. (1993). Identification and characterization of dinucleotide repeat (CA)n markers for genetic mapping in dog. Genomics 16, 207–213.
Roy, M.S., Geffen, E., Smith, D., Ostrander, E.A., Wayne, R.K. (1994). Patterns of differentiation and hybridization in North American wolflike canids revealed by analysis of microsatellite loci. J. Mol. Biol. Evol. 11, 553–570.
Tautz, D., Renz, M. (1984). Simple sequences are uniquitous repetitive components of eukaryotic genomes. Nucleic Acids Res. 12, 4127–4138.
Weber, J.L. (1990). Informativeness of human (dC-dA) n ·(dG-dT) n polymorphisms. Genomics 7, 524–530.
Weber, J.L., May P.E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44, 388–396.
Weissenbach, J., Gyapay, G., Dib, C., Vignal, A., Morissette, J., Millasseau, P., Vaysseix, G., M. Lathrop (1992). A second generation linkage map of the human genome. Nature 359, 794–801.
Wintero, A.K., Fredholm, M., Thomsen, P.D. (1992).Variable (dG-dT)n·(dC-dA)n sequences in the porcine genome. Genomics 12, 281–288.
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The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the accession numbers indicated in Table 1.
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Ostrander, E.A., Mapa, F.A., Yee, M. et al. One hundred and one new simple sequence repeat-based markers for the canine genome. Mammalian Genome 6, 192–195 (1995). https://doi.org/10.1007/BF00293011
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DOI: https://doi.org/10.1007/BF00293011