Summary
Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.
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Communicated by H. F. Linskens
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de Laat, A.M.M., Blaas, J. Flow-cytometric characterization and sorting of plant chromosomes. Theoret. Appl. Genetics 67, 463–467 (1984). https://doi.org/10.1007/BF00263414
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DOI: https://doi.org/10.1007/BF00263414