Summary
In this study, a Hertwig effect with a non-typical biphasic curve was obtained using sperm irradiated with increasing intensities of UV. The first phase of the UV curve appeared to be quite different from that normally demonstrated using γ or x-ray irradiation. This difference is characterised throughout the length of the first phase by (1) low and stable embryo hatching rates of about 3.5%, and (2) exclusive formation of haploid embryos at any irradiation intensity. Additionally, at both phases, the ability of the sperm to induce morula formation was not affected at all, and no aneuploidy nor chromosomal fragments could be seen. Therefore, it was suggested that in this fish the lethal effect of UV irradition on sperm is mainly expressed on early differentiative events during embryogenesis, which lead to a degeneration of the embryos during early stages of their development. The possible mechanism by which haploidy is achieved during the first phase is discussed. Two generations of diploid gynogenetic tilapias were induced by activating Oreochromis aureus eggs with UV-irradiated O. niloticus sperm and by using the heat-shock technique, at optimized conditions, for the prevention of the second polar body extrusion. Species specific dominant genetic markers (serum esterases and tail striation) were used to confirm the exclusive content of the maternal genome in gynogenetic offspring. Very low survival rates (0.36%) were shown in F1 gynogenetic fish as well as a high incidence of malformations among survivors. In the second gynogenetic generation, both significantly higher survival rates (3.6%) and a considerably reduced incidence of malformations were obtained. We suggest that low frequencies of recombination occur in this species and cause a rapid increase in the inbreeding level. This is followed by the expression of lethal and defective genes that are considerably reduced after second generation selection.
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References
Allendorf FW, Thorgaard GH (1984) Tetraploidy and the evolution of salmonid fish. In: Turner BJ (ed) Evolutionary genetics of fishes. Plenum Press, New York, pp 1–53
Avtalion RR (1982) Genetic markers in Sarotherodon and their use for sex and species identification. In: Pullin RSV, LoweMcConnell RH (eds) The biology and culture of tilapia. ICLARM, pp 269–277
Avtalion RR, Don J, Reich L (1988) Scale transplantation in gynogenetic and normal tilapias. Les colloques de l'INRA 44:213–220
Cherfas NB (1981) Gynogenesis in fish. In: Kirpichnikov VS (ed) Genetic bases of fish selection. Springer, Berlin Heidelberg New York, pp 255–273
Chourrout D (1982) Gynogenesis caused by ultraviolet radiation of salmonid sperm. J Exp Zool 223:175–181
Chourrout D (1984) Pressure induced retention of second polar body and suppression of first cleavage in rainbow trout: production of all triploid and heterozygous and homozygous diploid gynogenetics. Aquaculture 36:111–126
Chourrout D, Itskovich J (1983) Three manipulations permitted by artificial insemination in Tilapia: induced diploid gynogenesis, production of all triploid populations and intergeneric hybridization. In: Int Symp on Tilapia in aquaculture. Nazareth, Israel, pp 246–255
Chourrout D, Quillet E (1982) Induced gynogenesis in the rainbow trout: sex and survival of progenies, production of alltriploid populations. Theor Appl Genet 63:201–205
Chourrout D, Chevassus B, Herioux F (1980) Analysis of an “Hertwig effect” in the rainbow trout (Salmo gairdneri Richardson) after fertilization with irradiated sperm. Reprod Nutr Develop 20:719–726
Don J, Avtalion RR (1986) The induction of triploidy in Oreochromis aureus by heat shock. Theor Appl Genet 72: 186–192
Don J, Avtalion RR (1988) Ploidy and gynogenesis in tilapias. Les colloques de l'INRA 44:199–205
Don J, Koiller M, Yeheskel O, Avtalion RR, Nussbaum KE (1987) Increased Tilapia embryo viability using ultraviolet irradiation in a closed recirculating zuger-bottle system. Aquacult Engin 6:69–74
Guyomard R (1984) High level of residual heterozygosity in gynogenetic rainbow trout, Salmo gairdneri, Richardson. Theor Appl Genet 67:307–316
Hertwig O (1911) Die Radiumkrankheit tierischer Keimzellen, ein Beitrag zur experimentellen Zeugungs und Vererbungslehre. Arch Mikrosk Anat Entwicklungsmech 77 (2):97–164
Ijiri KI (1980) Gamma-ray irradiation of the sperm of the fish Oryzias latipes and induction of gynogenesis. J Radiat Res 21:263–270
Ijiri KI (1983) Chromosomal studies on radiation-induced gynogenesis and diploid gynogenesis in the fish Oryzias latipes. J Radiat Res 24:184–195
Ijiri KI, Egami N (1980) “Hertwig effect” cause by UVirradiation of sperm Oryzias latipes (Teleost) and its photoreactivation. Mutat Res 69:241–248
Koiller M, Avtalion RR (1985) A laboratory scale recycling water unit for Tilapia breeding. Aquacult Engin 4:235–246
Lasher R, Rugh R (1962) The “Hertwig effect” in Teleost development. Biol Bull 123:582–588
Mires D (1982) A study of the problems of the mass production of hybrid tilapia fry. In: Pullin RSV, Lowe-McConnell RH (eds) The biology and culture of tilapias. ICLARM, pp 317–329
Nagy A, Csanyi V (1984) A new breeding system using gynogenesis and sex-reversal for fast inbreeding in carp. Theor Appl Genet 67:485–490
Nagy A, Rajki K, Horvath L, Csanyi V (1978) Investigation on carp, Cyprinus carpio L. gynogenesis. J Fish Biol 13:214–224
Onozato H (1982) The “Hertwig effect” and gynogenesis in Chum salmon Oncorhynchus keta egs fertilized with 60Co γ ray irradiated milt. Bull Jpn Soc Fish 48:1237–1244
Onozato H (1984) Diploidization of gynogenetically activated salmon eggs using hydrostatic pressure. Aquaculture 43:91–97
Penman DJ, Shah MS, Beardmore JA, Skibinski DOF (1986) Sex ratios of gynogenetic and triploid Tilapia. EIFAC/FAO Symposium on Selection Hybridisation and Genetic Engineering in Aquaculture and Stocking, Bordeaux, France
Pogany GC (1971) Effect of sperm ultraviolet irradiation on the embryonic development of Rana pipiens. Dev Biol 26:336–345
Purdom CE (1969) Radiation-induced gynogenesis and androgenesis in fish. Heredity 24:431–444
Refstie T, Stoss J, Donaldson EM (1982) Production of all female Coho salmon (Oncorhynchus kisutch) by diploid gynogenesis using irradiated sperm and cold shock. Aquaculture 29:67–82
Schultz RJ (1967) Gynogenesis and triploidy in the viviparous fish Poeciliopsis. Science 157:1564–1566
Stanley JG (1979) Monosex and sterile fish for transplantation and aquaculture. Int Council for the Exploration of the Sea, Mariculture Committee. Warsaw, Poland
Streisinger G, Walker C, Dower N, Knauber D, Singer F (1981) Production of clones of homozygous diploid zebra fish (Bra chydanio rerio). Nature 291:293–296
Suzuki R, Oshiro T, Nakanishi T (1985) Survival, growth and fertility of gynogenetic diploids induced in the Cyprinid loach, Misgurnus anguillicaudatus. Aquaculture 48:45–55
Thorgaard GH, Scheerer PD, Parsons JD (1985) Residual paternal inheritance in gynogenetic rainbow trout: implications for gene transfer. Theor Appl Genet 71:119–121
Yeheskel O, Avtalion RR (1988) Artificial fertilization of tilapia eggs, a preliminary study. Les colloques de l'INRA 44: 169–175
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Communicated by E. J. Eisen
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Don, J., Avtalion, R.R. Production of F1 and F2 diploid gynogenetic tilapias and analysis of the “Hertwig curve” obtained using ultraviolet irradiated sperm. Theoret. Appl. Genetics 76, 253–259 (1988). https://doi.org/10.1007/BF00257853
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DOI: https://doi.org/10.1007/BF00257853