Summary
Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indicated that Mol7 possesses greater potential than B73 to acquire thermal tolerance. Poly(A)+ RNA, extracted from leaf blades, was translated in vitro in the presence of 35S-methionine and the translation products separated by twodimensional gel electrophoresis. Major genotypic differences were observed in the translation products. Mo 17 synthesized twelve unique heat shock proteins in the 15–18 kD range, but B73 synthesized only three unique heat shock proteins in the same range. DNA polymorphisms were observed between the maize lines using 32P labeled heat shock protein gene probes.
Similar content being viewed by others
Abbreviations
- HKT:
-
Heat-killing time
- HS:
-
Heat shock
- HSP:
-
Heat shock protein
- HMW:
-
High molecular weight
- LMW:
-
Low molecular weight
References
Atkinson BG, Liu L, Goping IN, Walden DB (1989) Expression of the genes encoding hsp73, hsp18, and ubiquitin in radicles of heatshocked maize seedlings. Genome 31:698–704
Baszczynski CL, Walden DB (1982) Regulation of gene expression in corn (Zea mays L.) by heat shock. Can J Biochem 60:569–579
Chaisompongpan N, Li PH, Davis DW, Markhart AH III (1990) Photosynthetic responses to heat stress in common bean genotypes differing in heat acclimation potential. Crop Sci 30:100–104
Chen H-H, Shen Z-Y, Li PH (1982) Adaptibility of crop plants to high temperature stress. Crop Sci 22:719–725
Cooper P, Ho T-HD (1983) Heat shock proteins in maize. Plant Physiol 71:215–222
Cooper P, Ho T-HD, Hauptmann RM (1984) Tissue specificity of the heat-shock response in maize. Plant Physiol 75:431–441
Cooper P, Ho T-HD (1987) Intracellular localization of heat shock proteins in maize. Plant Physiol 84:1197–1203
Dietrich, PS, Bouchard RA, Casey ES, Sinibaldi RM (1991) Isolation and characterization of a small heat shock protein gene from maize. Plant Physiol 96:1268–1276
Fender, SE, O'Connell MA (1989) Heat shock protein expression in thermotolerant and thermosensitive lines of cotton. Plant Cell Reports 8:37–40
Frova C, Taramino G, Binelli G, Ottaviano E (1988) Heat-shock protein variability in maize. Maydica 33:65–76
Goping IS, Frappier JR, Walden DB, Atkinson BG (1991) Sequence, identification and characterization of cDNA encoding two different members of the 18kDa heat shock family of Zea mays L. Plant Mol Biol 16:699–711
Krishnan M, Nguyen HT, Burke JJ (1989) Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol 90:140–145
Lin CY, Chen YM, Key JL (1985) Solute leakage in soybean seedlings under various heat shock regimes. Plant Cell Physiol 26(8):1493–149
Mans RJ, Novelli GD (1961) Measurement of the incorporation of radioactive amino acids into protein by a filter paper disk method. Arch Biochem Biophys 94:48–53
Nguyen HT, Krishnan M, Burke JJ, Porter DR, Vierling RA (1989) Genetic diversity of heat shock protein synthesis in cereal plants. In: Cherry JH (ed) Environmental stress in plants. Springer-Verlag Berlin Heidelberg, pp319–330
Nieto-Sotelo J, Vierling E, Ho T-HD (1990) Cloning, sequence analysis, and expression of a cDNA encoding a plastid-localized heat shock protein in maize. Plant Physiol 93:1321–1328
Riabowol KT, Mizzen LA, Welch WJ (1988) Heat shock is lethal to fibroblasts microinjected with antibodies against HSP70. Science 242:433–436
Rochester DF, Winer JA, Shah DM (1986) The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO 5(3):451–458
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning A Laboratory Manual 2nd edn. Cold Spring Harbor Laboratory Press
Sanchez Y, Lindquist SL (1990) HSP104 Required for induced thermotolerance. Science 248:1112–1115
Steel RGD, Torrie JH (1980) Principles and procedures of statistics. A biometrical approach. 2nd ed. Mc-Graw-Hill New York pp239–271
Vierling E (1991) The roles of heat shock proteins in plants. Annu Rev Plant Phys Plant Mol Biol 42:579–620
Vierling RA, Nguyen HT (1990) Heat-shock protein synthesis and accumulation in diploid wheat. Crop Sci 30:1337–1342
Yacoob RK, Filion WG (1986) Temperature-stress response in maize: a comparison of several cultivars. Can J Genet Cytol 28:1125–1131
Author information
Authors and Affiliations
Additional information
Communicated by J. M. Widholm
Contribution of the College of Agricultural Sciences, Texas Tech University, Journal No. T-4-333
Rights and permissions
About this article
Cite this article
Jorgensen, J.A., Weng, J., Ho, Th.D. et al. Genotype-specific heat shock proteins in two maize inbreds. Plant Cell Reports 11, 576–580 (1992). https://doi.org/10.1007/BF00233096
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00233096