Summary
In order to aim at a proved pretreatment for the enzymatic saccharification of lignocellulosic materials, the combined effect of the steam explosion treatment and the newly added alkali-hydrogen peroxide treatment was investigated. As a result, the following findings were obtained from comparison of the saccharification ratio (%) among the materials used: 1) Marked differences based on the lignocellulosic species characteristic of biological materials were observed. 2) Lignocellulosic samples such as rice straw, poplar, and maple chips gave enzymatic saccharification ratio (%) close to 100% by using the samples obtained after extraction with various solvents without alkali-hydrogen peroxide treatment. 3) Chip samples such as Japanese larch, loblolly pine, and Japanese cypress gave the very low enzymatic saccharification ratio (%) of 20–40% after the steam explosion and extraction with various solvents. 4) In comparison with enzymatic saccharification ratio (%) of original samples treated with steam explosion, alkali-hydrogen peroxide treatment resulted in greater effect based on the treatment of 2–2.5 times. 5) Alkali-hydrogen peroxide treatment afforded remarkable effects based on the treatment of the softwoods, such as Japanese larch and loblolly pine.
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The author is indebted to Professor Mitsuhiko Tanahashi, Faculty of Agriculture, Gifu University, for assistance on preparation of various lignocellulosic samples using a steam explosion equipment. The investigation was supported by the grant-in-aid for scientific research of the Ministry of Education in Japan
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Maekawa, E. On an available pretreatment for the enzymatic saccharification of lignocellulosic materials. Wood Sci.Technol. 30, 133–139 (1996). https://doi.org/10.1007/BF00224965
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DOI: https://doi.org/10.1007/BF00224965