Abstract
The formation of histamine from its precursor histidine is catalyzed by histidine decarboxylase (HDC), a pyridoxal phosphate (PLP)-dependent decarboxylase. The knowledge of sequence similarities between various rodent HDCs permitted us to prepare a rat brain HDC cDNA probe. After reverse transcription of rat brain polyA + mRNA, the HDC cDNA obtained was amplified by the polymerase chain reaction using two specific primers. The resulting 1019-bp DNA was cloned in the p-MAL vector. Its sequence corresponds to the published data on rat fetal liver HDC. This 1019-bp rat probe detected two BamHI sequences in man; these were assigned to chromosome 15 by somatic hybrid cell analysis. According to the well-known homology between human chromosome 15 and mouse chromosome 2, the result obtained is in agreement with the published localization of HDC on mouse chromosome 2. The mapping of the human HDC gene on chromosome 15 is an original contribution to the chromosomal assignment of related PLP-dependent decarboxylases.
Similar content being viewed by others
References
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Bruneau G, Gross MS, Krieger M, Bernheim A, Thibault J, Nguyen VC (1990) Preparation of a human dopa decarboxylase cDNA probe by PCR and its assignment to chromosome 7. Ann Génét 33:208–213
Chirgwin JM, Przybla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
Chudomelka PJ, Ramaley RF, Murrin LC (1990) Histidine decarboxylase from rat and rabbit brain: partial purification and characterization. Neurochem Res 15:17–24
Dutrillaux B, Lejeune J (1971) Sur une nouvelle technique d'analyse du caryotype humain. CR Acad Sci [III] 272:2638–2640
Human Gene Mapping 10 (1989) 10th International Workshop on Human Gene Mapping. Cytogenet Cell Genet 51:1–1147
Jackson FR (1990) Prokaryotic and eukaryotic pyridoxal-dependent decarboxylases are homologous. J Mol Evol 31:325–329
Joseph DR, Sullivan PM, Wang Y, Kozak C, Fenstermacher DA, Behrendsen ME, Zahnon CA (1990) Characterization and expression of the complementarv DNA encoding rat histidine decarboxylase. Proc Natl Acad Sci USA 87:733–737
Karlsen AE, Hagopian WA, Grubin CE, Dube S, Disteche CM, Adler DA, Barmeier CE, Mathewes S, Grant FJ, Foster D, Lenmark A (1991) Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10. Proc Natl Acad Sci USA 88:8337–8341
McGeer PE, Eccles JC, McGeer EG (1987) Molecular neurobiology of the mammalian brain, 2nd edn. Plenum Press, New York
Nguyen VC, Weil D, Fainz C, Cohen-Hagenauer O, Gross MS, Jegou-Foubert C, Tand MF de, Cochet C, Grouchy J de, Frezal J (1986) Panel of twenty-five independent man-rodent hybrids for human genetic marker mapping. Ann Genet 29:20–26
Ohmori E, Fukui T, Imanishi N, Yatsunami K, Ichiwaka A (1990) Purification and characterization of E-histidine decarboxylase from mouse mastocytoma P-815 cells. J Biochem 107:834–839
Savany A, Cronenberger L (1982) Properties of histidine decarboxylase from rat gastric mucosa. Eur J Biochem 12:593–599
Sparkes RS, Kaufman DE, Heinzmann C, Tobin AJ, Mohandas T (1987) Brain glutamate decarboxylase (GAD) gene assigned to human chromosome 2 by somatic cell hybrid analysis. Cytogenet Cell Genet 46:696(A0840)
Steinbusch HWM (1991) Distribution of histaminergic neurons and fibers in rat brain. Acta Otolaryngol (Stockh) 479 [Suppl]:12–23
Taguchi Y, Watanabe T, Kubota H, Hayashi H, Wada H (1984) Purification of histidine decarboxylase from the liver of fetal rats and its immunochemical and immunohistochemical characterization. J Biol Chem 259:5214–5221
Van Someren H, Van Henegouven HB, Los W, Wurzer-Figurelli E, Doppert B, Vervloet M, Meera Khan P (1974) Enzyme electrophoresis on cellulose acetate gel. Hum Genet 25:189–201
Wada H, Inagaki N, Yamatodani A, Watanabe T (1991) Is the histaminergic neuron system a regulatory center for whole-brain ctivity? Trends Neurosci 14:415–418
Yamamoto J, Yatsunami K, Ohmori E, Sugimoto Y, Fuki T, Katayama T, Ichikawa A (1990) cDNA-derived amino acid sequence of L-histidine decarboxylase from mouse mastocytoma P-815 cells. FEBS Eett 276:214–218
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bruneau, G., Van Nguyen, C., Gros, F. et al. Preparation of a rat brain histidine decarboxylase (HDC) cDNA probe by PCR and assignment of the human HDC gene to chromosome 15. Hum Genet 90, 235–238 (1992). https://doi.org/10.1007/BF00220068
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00220068