Summary
Dichloromethylene diphosphonate can be used for temporary elimination of macrophages in the spleen when administered after entrapment in liposomes. No comparable effect on the macrophages of the spleen was observed with free dichloromethylene diphosphonate or in the case of empty liposomes. Marginal metallophils on the boundary between white pulp and marginal zone as well as macrophages in the marginal zone and red pulp disappeared from the spleen within one day and remained largely absent for about a week. After this time cells reappeared slowly, and at approximately four weeks after injection their presence in the spleen did not differ from that in control animals. Marginal metallophils and macrophages in the spleen were demonstrated by use of enzyme-histochemical methods and by their capacity to ingest carbon particles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Davis BJ, Ornstein L (1959) High resolution enzyme localization with a new diazo reagent ‘hexazonium pararosaniline’. J Histochem Cytochem 7:297–298
Eikelenboom P (1978a) Characterization of non-lymphoid cells in the white pulp of the mouse spleen: an in vivo and in vitro study. Cell Tissue Res 195:445–460
Eikelenboom P (1978b) Dendritic cells in the rat spleen follicles. A combined immuno- and enzyme histochemical study. Cell Tissue Res 190:79–87
Eikelenboom P, Dijkstra CD, Boorsma DM, Van Rooijen N (1984) Characterization of lymphoid and non-lymphoid cells in the white pulp of the spleen using immunohistoperoxidase techniques and enzyme-histochemistry. Experientia (in press)
Gregoriadis G, Senior J, Trouet A (1982) Targeting of drugs, NATO Adv. Study Institute series A, vol 47, Plenum Press, New York
Groeneveld PHP, Eikelenboom P, Van Rooijen N (1983) Mechanisms of follicular trapping: similarities and differences in trapping of antibody-complexed antigens and carbon particles in the follicles of the spleen. J Reticuloendothel Soc 33:109–117
Groeneveld PHP, Van Rooijen N, Eikelenboom P (1983) In vivo effects of lipopolysaccharide on lymphoid and non-lymphoid cells in the mouse spleen. Migration of marginal metallophils towards the follicle centres. Cell Tissue Res 234:201–208
Juliano RL (1982) Liposomes and the reticuloendothelial system: interactions of liposomes with macrophages and behavior of liposomes in vivo. In: Targeting of drugs. (Gregoriadis G et al. eds) NATO Adv Study Institute, vol 47, Plenum Press, New York, p 285
Jung A, Chantraine A, Donath A, Van Ouwenaller C, Turnill D, Mermillod B, Kilter ME (1983) Use of dichloromethylene diphosphonate in metastatic bone disease. New Engl J Med 308:1499–1501
Reitsma PH (1982) Differential actions of diphosphonates on bone resorbing cells and the adjustment of skeletal metabolism. Academic thesis, drukkerij JH Pasmans BV, The Hague, The Netherlands
Ryman BE, Tyrrell DA (1980) Liposomes — bags of potential. Essays Biochem 18:49–98
Steinman RM, Cohn ZA (1973) Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation and tissue distribution. J Exp Med 137:4142
Unanue ER (1981) The regulatory role of macrophages in antigenic stimulation. Part two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol 31:1–136
Van Furth R (1980) Mononuclear phagocytes, Part I and II. Martinus Nijhoff Publishers, The Hague, The Netherlands
Van Rooijen N (1972) The vascular pathways in the white pulp of the rabbit spleen. Acta Morphol Neerl-Scand 10:351–357
Van Rooijen N, Roeterink CH (1980) Phagocytosis and lymphocyte migration: evidence that lymphocyte trapping in the spleen following carbon injection is not due to direct lymphocyte-macrophage adherence. Immunology 39:571–576
Van Rooijen N, Van Nieuwmegen R (1983) Use of liposomes as biodegradable and harmless adjuvants. Meth Enzym 93:83–95
Weiss L (1964) The white pulp of the spleen. The relationships of arterial vessels, reticulum and free cells in the periarterial lymphatic sheath. Bull Johns Hopk Hosp 115:99–173
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van Rooijen, N., van Nieuwmegen, R. Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. Cell Tissue Res. 238, 355–358 (1984). https://doi.org/10.1007/BF00217308
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00217308