Abstract
We have developed a rapid and sensitive assay for the detection of clostridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) sequences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plate. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clostridial cells or spores in experimentally contaminated soft drinks.
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Correspondence to: J. L. Ramírez
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Galindo, I., Rangel-Aldao, R. & Ramírez, J.L. A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of Clostridium spp.. Appl Microbiol Biotechnol 39, 553–557 (1993). https://doi.org/10.1007/BF00205050
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DOI: https://doi.org/10.1007/BF00205050