Skip to main content
Log in

Assignment of the gene coding for human cytochrome c oxidase subunit VIb to chromosome 19, band q13.1, by fluorescence in situ hybridisation

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A cloned, 40 kb, genomic DNA fragment, containing the last exon of the gene for human cytochrome c oxidase subunit VIb and its flanking sequences, was used as a probe to localize the subunit VIb gene on human metaphase chromosomes. The probe was labelled with Bio-11-dUTP and detected by fluorescence. Subsequent R-banding indicated that the cytochrome c oxidase subunit VIb gene is localized in band 19q13.1, extending the evidence that the human nuclear genes of cytochrome c oxidase are not clustered.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson S, Bankier AT, Barrell BG, De Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Sequence and organisation of the human mitochondrial genome. Nature 290:457–465

    CAS  PubMed  Google Scholar 

  • Bates PF, Swift RA (1983) Double cos site vectors: simplified cosmid cloning. Gene 26:137–146

    Google Scholar 

  • Bisson R, Schiavo G, Montecucco C (1987) ATP induces conformational changes in mitochondrial cytochrome c oxidase. J Biol Chem 26:4373–4376

    Google Scholar 

  • Capaldi RA (1990) Structure and function of cytochrome c oxidase. Annu Rev Biochem 59:569–596

    Google Scholar 

  • Cherif D, Julier C, Delattre O, Derré J, Lathrop GM, Berger R (1990) Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy: application to regional mapping of hman chromosome 11. Proc Natl Acad Sci USA 87:6639–6643

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    CAS  PubMed  Google Scholar 

  • Hatefi Y (1985) The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem 54:1015–1069

    Article  CAS  PubMed  Google Scholar 

  • Kadenbach B (1986) Regulation of respiration and ATP synthesis in higher organisms: hypothesis. J Bioenerg Biomembr 18:39–54

    Google Scholar 

  • Kadenbach B, Kuhn-Nentwig L, Büge U (1987) Evolution of a regulatory enzyme: cytochrome c oxidase (complex IV). In: Lee CP (ed) Current topics in bioenergetics, vol 15. Academic Press, New York, pp 113:161

    Google Scholar 

  • Kievits T, Dauwerse JG, Wiegant J, Devilee P, Breuning MH, Cornelisse CJ, Van Ommen G-JB (1990) Rapid subchromosomal localization of cosmids by nonradioactive in situ hybridization. Cytogenet Cell Genet 53:134–136

    Google Scholar 

  • Lomax MI, Grossman LI (1989) Tissue-specific genes for respiratory proteins. Trends Biochem Sci 14:501–503

    Google Scholar 

  • Lomax MI, Welch MD, Darras BT, Francke U, Grossman LI (1990) Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunit IV. Gene 86:209–216

    Google Scholar 

  • Poyton RO, Trueblood CE, Wright RM, Farrell LE (1988) Expression and function of cytochrome c oxidase subunit isologues — modulators of cellular energy production? Ann NY Acad Sci 550:289–307

    Google Scholar 

  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA (1989) A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem 264:10595–10600

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Taanman J-W, Schrage C, Ponne N, Bolhuis P, De Vries H, Agsteribbe E (1989) Nucleotide sequence of cDNA encoding subunit VIb of human cytochrome c oxidase. Nucleic Acids Res 17:1766

    Google Scholar 

  • Taanman J-W, Schrage C, Ponne NJ, Das AT, Bolhuis PA, De Vries H, Agsteribbe E (1990) Isolation of cDNAs encoding subunit VIb of human cytochrome c oxidase and steady-state levels of coxVIb mRNA in different tissues. Gene 93:285–291

    Google Scholar 

  • Taanman J-W, Schrage C, Reuvekamp P, Bijl J, Hartog M, De ries H, Agsteribbe E (1991) Identification of three human pseudogenes for subunit VIb of cytochrome c oxidase: a molecular record of gene evolution. Gene (in press)

  • Yamamura W, Zhang Y-Z, Takamiya S, Capaldi RA (1988) Tissue-specific differences between heart and liver cytochrome c oxidase. Biochemistry 27:4909–4914

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taanman, JW., van der Veen, A.Y., Schrage, C. et al. Assignment of the gene coding for human cytochrome c oxidase subunit VIb to chromosome 19, band q13.1, by fluorescence in situ hybridisation. Hum Genet 87, 325–327 (1991). https://doi.org/10.1007/BF00200913

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00200913

Keywords

Navigation