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Effects of centrifugation on preprophase-band formation in Adiantum protonemata

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Abstract

Protonemata of Adiantum capillus-veneris L., grown in red light and consisting of a single elongate cell, were centrifuged at various times after or just before the induction of synchronous cell division by irradiation with blue light (BL), and formation and location of preprophase bands (PPBs) of microtubules (MTs) were observed by immunofluorescence microscopy. The cells divided approx. 14–20 h after the onset of BL irradiation, irrespective of the timing of centrifugation. Basipetal centrifugation caused basipetal displacement (about 100 μm) of the nucleus. The nucleus did not return to its original position and cell division occurred at the new nuclear position. A PPB was formed in the region of the displaced nucleus except in some cells which presumably had been centrifuged just before cell division. Microtubule behavior in the apical region, where cell division occurred in non-centrifuged cells, depended on the time of centrifugation relative to the cell cycle. When protonemata were centrifuged before PPB formation, no PPB was formed in the apical region of most cells but, in some cells, another MT band indistinguishable from a PPB (we consider it PPB) was formed at this site after the displacement of the nucleus. When protonemata were centrifuged during or after PPB formation, the PPB, which had already formed in the apical region, did not disappear until the sedimented nucleus entered telophase or early interphase, while a new PPB was formed in most cells in the region of the displaced nucleus. These cells had thus two PPBs: one in the apical region, and another in the region where the displaced nucleus was located. Double centrifugation to displace the nucleus twice showed that once a PPB formed, irrespective of its intracellular position, it remained after the displacement of the nucleus. However, no PPBs were observed in the region where the nucleus was located after the first centrifugation if the nucleus was again displaced before a PPB had formed in this region. The positioning of the cell plate was also examined. When centrifugation was performed just before cell division, PPB formation around the displaced nucleus decreased and oblique cell plates were observed at an increased frequency. In conclusion, preprophase nuclei may have the ability to induce PPB formation in the neighboring cell cortex, and PPBs, in turn, may have a role in governing the orientation of cell plates.

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Abbreviations

BL:

blue light

DAPI:

4′,6′-diamidino-2-phenylindole

MT:

microtubule

PPB:

preprophase band

References

  • Brown, R.C., Lemmon, B.E. (1988) Preprophasic microtubule systems and development of the mitotic spindle in hornworts (Bryophyta). Protoplasma 143, 11–21

    Google Scholar 

  • Cho, S.-O., Wick, S.M. (1989) Microtubule orientation during stomatal differentiation in grasses. J. Cell Sci. 92, 581–594

    Google Scholar 

  • Galatis, B., Apostolakos, P., Katsaros, C. (1984) Experimental studies on the function of cortical cytoplasmic zone of the preprophase microtubule band. Protoplasma 122, 11–26

    Google Scholar 

  • Gunning, B.E.S. (1982) The cytokinetic apparatus: Its development and spatial regulation. In: The cytoskeleton in plant growth and development, pp. 229–292, Lloyd, C.W., ed. Academic Press, London

    Google Scholar 

  • Gunning, B.E.S., Hardham, A.R., Hughes, J.E. (1978) Pre-prophase bands of microtubules in all categories of formative and proliferative cell division in Azolla roots. Planta 143, 145–160

    Google Scholar 

  • Lloyd, C.W. (1987) The plant cytoskeleton: The impact of fluorescence microscopy. Annu. Rev. Plant Physiol. 38, 119–139

    Google Scholar 

  • Mineyuki, Y., Wick, S.M., Gunning, B.E.S. (1988) Preprophase bands of microtubules and cell cycle: Kinetics and experimental uncoupling of their formation from the nuclear cycle in onion root-tip cells. Planta 174, 518–526

    Google Scholar 

  • Mineyuki, Y., Marc, J., Palevitz, B.A. (1989) Development of the preprophase band from random cytoplasmic microtubules in guard mother cells of Allium cepa L. Planta 178, 291–296

    Google Scholar 

  • Miyata, M., Wada, M., Furuya, M. (1979) Effects of phytochrome and blue-near ultraviolet light-absorbing pigment on duration of component phases of the cell cycle in Adiantum gametophytes. Develop. Growth Different. 21, 577–584

    Google Scholar 

  • Murata, T., Wada, M. (1989) Re-organization of microtubules during preprophase band development in Adiantum protonemata. Protoplasma 151, 73–80

    Google Scholar 

  • Ôta, T. (1961) The role of cytoplasm in cytokinesis of plant cells. Cytologia 26, 428–447

    Google Scholar 

  • Pickett-Heaps, J.D. (1969) Preprophase microtubules and stomatal differentiation; Some effects of centrifugation on symmetrical and asymmetrical cell division. J. Ultrastruct. Res. 27, 24–44

    Google Scholar 

  • Schmiedel, G., Schnepf, E. (1980) Polarity and growth of caulonema tip cells of moss Funaria hygrometrica. Planta 147, 405–413

    Google Scholar 

  • Simmonds, D.H. (1986) Prophase bands of microtubules occur in protoplast cultures of Vicia hajastana Grossh. Planta 167, 469–472

    Google Scholar 

  • Wada, M., O'Brien, T.P. (1975) Observations on the structure of the protonema of Adiantum capillus-veneris L. undergoing cell division following white-light irradiation. Planta 126, 213–227

    Google Scholar 

  • Wada, M., Mineyuki, Y., Kadota, A., Furuya, M. (1980) The change of nuclear position and distribution of circumferentially aligned cortical microtubules during the progression of cell cycle in Adiantum protonemata. Bot. Mag. Tokyo 93, 237–245

    Google Scholar 

  • Wada, M., Kadota, A., Furuya, M. (1983) Intracellular localization and dichroic orientation of phytochrome in plasma membrane and/or ectoplasm of a centrifuged protonema of fern Adiantum capillus-veneris L. Plant Cell Physiol. 24, 1441–1447

    Google Scholar 

  • Wick, S.M., Duniec, J. (1983) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Preprophase band development and concomitant appearance of nuclear envelope-associated tubulin. J. Cell Biol. 97, 235–243

    Google Scholar 

  • Wick, S.M., Duniec, J. (1984) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. II. Transition between the pre-prophase band and the mitotic spindle. Protoplasma 122, 45–55

    Google Scholar 

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This work was supported in part by Grant-in-Aid for General Scientific Research (No. 01540567) from the Ministry of Education, Science and Culture in Japan. The authors are grateful to Dr. T.H. Giddings Jr. (University of Colorado, Boulder, USA) for critical reading of the manuscript.

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Murata, T., Wada, M. Effects of centrifugation on preprophase-band formation in Adiantum protonemata. Planta 183, 391–398 (1991). https://doi.org/10.1007/BF00197738

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