Abstract
Respiratory tract infections have been thought to act as a trigger mechanism in sudden infant death. In 118 autopsy cases of infant death, paraffin-embedded or frozen lung tissues were investigated by means of a nested polymerase chain reaction (PCR) to detect adenovirus (AV) DNA. The primers used are general primers and allow the detection of most pathogenic adenoviruses with high specificity and sensitivity and independently of devitalization of viruses or degradation of viral DNA. For the investigation three groups were established: there were 13 cases of unnatural death, 78 cases of natural death without histological signs of interstitial pneumonia, and 27 cases with interstitial pneumonia. The first group was AV negative. In the group without interstitial pneumonia AV was detected in 10.2% of the cases. In the group with interstitial pneumonia the frequency of AV detection was almost 26%. The results obtained demonstrate an association between interstitial pneumonia and detection of AV DNA, indicating that AV may play an important part in pulmonary infection in infants. Histological evidence of interstitial pneumonia was not observed in all AV-positive cases, perhaps because nonspecific virus-related changes occurred only in early stages of viral infection. Comparison of the AV frequency in SIDS (15%) and non-SIDS cases (4%) indicates an association between pulmonary AV infections and sudden death. These results support the working hypothesis of respiratory infections acting as a trigger mechanism in sudden infant death.
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References
Akusjärvi G, Aleström P, Pettersson M, Lager M, Jörnvall H, Pettersson U (1984) The gene for the adenovirus 2 hexon polypeptide. J Biol Chem 259: 13976–13979
Allard A, Girones R, Juto P, Wadell G (1990) Polymerase chain reaction for the detection of adenoviruses in stool samples. J Clin Microbiol 8: 2659–2667
An SF, Gould S, Keeling JW, Fleming KA (1993) Role of respiratory viral infection in SIDS: detection of viral nucleic acid by in situ hybridization. J Pathol (Lond) 171: 271–278
Bajanowski T, Wiegand P, Brinkmann B (1994) Comparison of different methods for CMV detection. Int J Legal Med 106: 219–222
Blackwell CC, Saadi AT, Raza MW, Weir DM, Busuttil A (1993) The potential role of bacterial toxins in Sudden Infant Death Syndrome (SIDS). Int J Legal Med 105: 333–338
Brooks DR, Krous HF, Burton JL, McKinley J, McKinley W (1994) Results of a pilot test of the global strategy workshop's international standardized autopsy protocol (SAP) in Georgia. In: Sudden infant death syndrome (SIDS), Third SIDS International Conference, Stavanger, Norway, 31.07.1994–04.08.1994, program and abstracts, p 155
Costa J, Rabson W (1985) Viral diseases. In: Kissane JM (ed) Anderson's pathology. Mosby, St Louis Toronto Princeton, pp 358–360
Coumbe A, Fox JD, Briggs M, Tedder RS, Berry CL (1990) Cytomegalovirus and human herpesvirus-6 in sudden infant death syndrome: an in-situ hybridization study. Pediatr Pathol 10: 483–490
DeJong JC, Wigand R, Kidd AH, Wadell G, Kapsenberg JG, Muzerie CJ, Wermenbol AG, Firtzlaff RG (1983) Candidate adenovirus 40 and 41: fastidious adenoviruses from human infant stool. J Med Virol 11: 215–231
Entrup M, Brinkmann B (1990) Histologische Lungenbefunde beim plötzlichen Kindstod. Z Rechtsmed 103: 425–433
Goodpasture EW, Auerbach SH, Swanson HS, Cotter EF (1939) Virus pneumonia of infants secondary to epidemic infections. Am J Dis Child 57: 997–1011
Hanna L, Jawetz E (1962) Attempts to enhance infectivity of adenovirus type 8 for cell culture. Proc Soc Exp Biol 110: 707–709
Horne RW, Brenner S, Waterson AP, Wildy P (1959) The icosahedral form of an adenovirus. J Mol Biol 1: 84–86
Jawetz E, Kimura S, Nicholas AN, Thygeson P, Hanna L (1955) New type of APC virus from keratoconjunctivitis. Science 122: 1190–1192
Kinloch R, Mackay N, Mautner V (1984) Adenovirus hexon. Sequence comparison of subgroup C serotypes 2 and 5. J Biol Chem 259: 6431–6436
Knipe DM (1990) Adenoviruses. In: Fields BN (ed) Virology. Raven Press, New York, p 1729
Möller A, Meyer E, Brinkmann B (1994) Different types of structural variation in STRs: HumFES/FPS, HumVWA and HUMD21S11. Int J Legal Med 106: 319–323
Philipson L (1983) Structure and assembly of adenoviruses. In: Doerfler W (ed) The molecular biology of adenoviruses. Springer, Berlin Heidelberg New York, pp 1–52
Pring-Åckerblom P, Adrian T (1993) The hexon genes of adenoviruses of subgenus C: comparison of the variable regions. Res Virol 144: 117–127
Pring-Åkerblom P, Adrian T (1994) Type and group-specific polymerase chain reaction for adenovirus detection. Res Virol 145: 25–35
Rowe WP, Huebner RJ, Gilmore LK, Parrott RH, Ward TG (1953) Isolation of a cyto-pathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med 84: 570–573
Schäfer HE (1983) Inflammatory diseases of the human lung of definite or presumed viral origin. Cytologic and histologic topics. In: Müller (ed) Pulmonary diseases. Springer, Berlin Heidelberg New York, pp 153–205
Spencer H (1985) Pneumonias due to rickettsiae, chlamydiae, viruses and mycoplasma. In: Spencer H (ed) Pathology of the lung Pergamon Press, Oxford New York, pp 213–259
Stoltenberg L, Saugstad OD, Rognum TO (1992) Sudden infant death syndrome victims show local immunoglobulin M response in tracheal and immunoglobulin A response in duodenal mucosa. Pediatr Res 31: 372–375
Strano AJ, Henson DE (1975) Fatal adenovirus pneumonia: a study of 17 cases (abstract). Lab Invest 31: 346
Uren EC, Williams AL, Jack I, Rees JW (1980) Association of respiratory virus infection with sudden infant death syndrome. Med J Aust 1: 417–419
Wadell G (1987) Adenoviruses. In: Zuckerman AJ, Banatvala JE, Patterson JR (eds) Principles and practise of clinical virology, Wiley, Chichester New York, pp 251–274
Wigand R, Gelderblom H, Özel M, Distler H, Adrian T (1983) Characteristics of mastadenovirus h8, the causative agent of epidemic keratoconjunctivitis. Arch Virol 76: 307–319
Williams AL, Uren EC, Bretherton L (1984) Respiratory viruses and sudden infant death. BMJ 288: 1491–1493
Wilske J (1993) Zur Definition und Pathophysiologie des plötzlichen Säuglingstodes. In: Kruse K, Oehmichen M (eds) Plötzlicher Säuglingstod. (Themen der Kinderheilkunde, vol 6) Hansisches Verlagskontor, Lübeck, pp 7–18
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Bajanowski, T., Wiegand, P., Cecchi, R. et al. Detection and significance of adenoviruses in cases of sudden infant death. Vichows Archiv A Pathol Anat 428, 113–118 (1996). https://doi.org/10.1007/BF00193939
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DOI: https://doi.org/10.1007/BF00193939