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Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system

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Abstract

The scopolamine-releasing hairy root clone DL47-1 of Duboisia leichhardtii was cultured in an Amberlite XAD-2 column-combined bioreactor system for continuous production of scopolamine. The medium used was continuously exchanged during culture to maintain the electrical conductivity of the medium constant. After culturing the hairy roots in the system for 11 weeks, 0.5 g/l of scopolamine was obtained in the column. When the roots were cultures in the reactor system containing polyurethane foam or stainless-steel mesh to support the hairy roots, scopolamine recovery was increased. Thereafter, a two-stage culture, the first stage in the medium for hairy root growth and the second stage in the medium for scopolamine release, was carried out in this system by using a turbine-blade reactor with stainless-steel mesh as a support. Under these conditions, 1.3 g/l of scopolamine was recovered during 11 weeks of culture in the medium for scopolamine release. This bioreactor system seems applicable for the production of various plant metabolites by cultures of hairy roots.

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Author notes

  1. Hideo Ohkawa

    Present address: Department of Plant Protection, Faculty of Agriculture, Kobe University, 657, Kobe, Japan

Authors and Affiliations

  1. Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co., Ltd., 2-1, 4-chome Takatsukasa, 665, Takarazuka Hyogo, Japan

    Toshiya Muranaka & Hideo Ohkawa

  2. Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan

    Yasuyuki Yamada

Authors
  1. Toshiya Muranaka
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  2. Hideo Ohkawa
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  3. Yasuyuki Yamada
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Correspondence to: T. Muranaka

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Muranaka, T., Ohkawa, H. & Yamada, Y. Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system. Appl Microbiol Biotechnol 40, 219–223 (1993). https://doi.org/10.1007/BF00170370

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  • DOI: https://doi.org/10.1007/BF00170370

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