Summary
Endo-1,4-β-glucanase and xylanase were produced by Trichoderma reesei immobilized on polyurethane foam using lactose as the main carbon source. The most porous carrier was found to be the best of those tested. The nitrogen source and KH2PO4 concentration of the production medium had a marked effect on culture pH during the course of fermentation and, consequently, on xylanase activity. An increase in lactose concentration from 7 to 27 g/l resulted in an increase in endoglucanase activity (max. 730 U/ml), xylanase activity (max. 3350 U/ml) and filter paper activity (max. 3.0 FPU/ml).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, M.J., Buchert, J. and Viikari, L. (1993). Appl. Microbiol. Biotechnol. 40, 224–229.
Bailey, M.J. and Nevalainen, K.M.H. (1981). Enzyme Microbiol. Technol. 3, 153–157.
Bailey, M.J. and Poutanen, K. (1989). Appl. Microbiol. Biotechnol. 30, 5–10.
Duff, S.J.B. (1988). Biotechnol. Bioeng. 31, 345–348.
Enari, T.-M. and Niku-Paavola, M.-L. (1987). CRC Crit. Rev. Biotechnol. 5, 67–87.
Esterbauer, H., Steiner, W., Labudova, I., Hermann, A. and Hayn, M. (1991). Bioresource Technol. 36, 52–65.
Frein, E.M., Montenecourt, B.S. and Eveleigh, D.E. (1982). Biotechnol. Lett. 4, 287–292.
Haapala, R. and Linko, S. (1993). Appl. Microbiol. Biotechnol. 40, 494–498.
IUPAC (1987). Pure and Appl. Chem. 59, 257–268.
Kumakura, M., Tamada, M., Kasai, N., Kaetsu, I. and Kanno, S. (1989). Biotechnol. Bioeng. 33, 1358–1362.
Linko, M., Markkanen, P., Bailey, M. and Leisola, M. (1978). In Bioconversion of Cellulosic Substances into Energy Chemicals and Microbial Protein (T.K. Ghose, ed.), SFIT, Zürich, pp. 329–350.
Linko, S., Haapala, R. and Linko, P. (1994). In Proceedings of 6th European Congress of Biotechnology (L. Alberghina, L. Frontali and P. Sensi, eds.), Florence, Italy, 11.–17. June 1993 (in press)
Mukataka, S., Kabayashi, N., Sato, S. and Takahashi, J. (1988). Biotechnol. Bioeng. 32, 760–763.
Persson, I., Tjerneld, F. and Hahn-Hägerdahl, B. (1991). Process Biochem. 26, 65–74.
Reese, E.T. and Ryu, D.Y. (1980). Enzyme Microbiol. Technol. 2, 239–240.
Türker, M. and Mavituna, F. (1987). Enzyme Microb. Technol. 9, 739–743.
Viikari, L., Sundqvist, J. and Kettunen, J., (1991). Paperi ja Puu - Paper and Timber 73, 384–389.
Webb, C., Fukuda, H. and Atkinson, B. (1986). Biotechnol. Bioeng. 28, 41–50.
Xin, L.Z. and Kumakura, M. (1993). Enzyme Microb. Technol. 15, 300–303.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Haapala, R., Linko, S., Parkkinen, E. et al. Production of endo-1,4-β-glucanase and xylanase by Trichoderma reesei immobilized on polyurethane foam. Biotechnol Tech 8, 401–406 (1994). https://doi.org/10.1007/BF00154311
Issue Date:
DOI: https://doi.org/10.1007/BF00154311