Abstract
In etiolated leaves the occurrence of the enzymatic violaxanthin de-epoxidation to zeaxanthin is shown. The carotenoid transformation is provoked by the infiltration of whole leaves with ascorbate at pH 5 and is susceptible to DTT. Identification of the de-epoxidase activity is achieved by in vivo spectroscopy and pigment analysis (TLC).
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Abbreviations
- DTT:
-
Dithiothreitol
- Hepes:
-
N-2-hydroxyethylpiperazine-N′-2 ethanesulfonic acid
- TLC:
-
Thin-layer Chromatography
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Pfündel, E., Strasser, R.J. Violaxanthin de-epoxidase in etiolated leaves. Photosynth Res 15, 67–73 (1988). https://doi.org/10.1007/BF00054989
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DOI: https://doi.org/10.1007/BF00054989