Skip to main content
Log in

The Daphnia bioassay: a critique

  • Published:
Hydrobiologia Aims and scope Submit manuscript

Abstract

Daphnia magna is used widely as a standard ecotoxicological indicator organism, and protocols exist for its use in assessing the toxicity of substances under acute and chronic experimental conditions. Problems exist in repeatability of such bioassays between laboratories. Sources of variation are identified using a simple quantitative genetics model. Presenting specific examples, we conclude that these problems are tractable, but only if the genotype and culture conditions prior to and during tests are strictly controlled.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adema, D. M. M., 1978. Daphnia magna as a test animal in acute and chronic toxicity tests. Hydrobiol. 59: 125–134.

    Google Scholar 

  • Berge, W. F. ten, 1978. Breeding Daphnia magna. Hydrobiol. 59: 121–123.

    Google Scholar 

  • Cabridenc, R., dy1986. Exercice d'intercalibration concernant une méthode de determination de l'ecotoxicité à moyen terme des substances chimiques vis-à-vis des daphnies. Unpublished EC Report. Contract W/63/476 (214). Ref. I.R.C.H.A.D. 8523. Vert-le-Petit, France. 20 pp.

  • Falconer, D. S., 1981. Introduction to quantitative genetics. Longman. Harlow, Essex. 340 pp.

    Google Scholar 

  • Goulden, C. E., R. M. Comotto, J. A. Hendrickson Jr., L. L. Horning & K. L. Johnson, 1982. Procedures and recommendations for the culture and use of Daphnia in bioassay studies. Pages 139–160 in Proceedings of the American Society for Testing and Materials Fifth Toxicology Symposium, Special Technical Publication Number 7668, Philadelphia, Pennsylvania, USA.

  • Hebert, P. D. N. & M. Beaton, 1986. Cellulose-acetate gel electrophoresis. Unpublished ms. Univ. Windsor, Ontario. 34 pp.

    Google Scholar 

  • OECD., 1981. Daphnia sp. 14 day reproduction test (including acute immobilisation test). OECD guidelines for testing of Chemicals, no. 202. ISBN 92-64-1221-4. Paris. 15 pp.

  • Tessier, A. J., L. L. Henry, C. E. Goulden & M. W. Durand, 1983. Starvation in Daphnia: Energy reserves and reproductive allocation. Limnol. Oceanogr. 28: 667–676.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baird, D.J., Barber, I., Bradley, M. et al. The Daphnia bioassay: a critique. Hydrobiologia 188, 403–406 (1989). https://doi.org/10.1007/BF00027806

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00027806

Key words

Navigation