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T-DNA insertional mutagenesis in Arabidopsis

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References

  1. Ambros PF, Matzke AJM, Matzke MA: Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization. EMBO J 5: 2073–2077 (1986).

    Google Scholar 

  2. André D, Colau D, Schell J, VanMontagu M, Hernalsteens J-P: Gene tagging in plants by a T-DNA insertion that generates APH(3′)II plant gene fusions. Mol Gen Genet 204: 512–518 (1986).

    Google Scholar 

  3. Ankenbauer RG, Best EA, Palanca CA, Nester EW: Mutants of Agrobacterium tumefaciens virA gene exhibiting acetosyringone-independent expression pattern of the vir regulon. Mol Plant-Microbe Interact 4: 400–406 (1991).

    Google Scholar 

  4. Baker B, Coupland G, Fedoroff N, Starlinger P, Schell J: Phenotypic assay for excision of the maize controlling element Ac in tobacco. EMBO J 6: 1547–1554 (1987).

    Google Scholar 

  5. Bilang R, Peterhans A, Bogucki A, Paszkowski J: Single-stranded DNA as recombination substrate in plants as assessed by stable and transient recombination assay. Mol Cell Biol 12: 329–336 (1992).

    Google Scholar 

  6. Castle LA, Smith KD, Morris RO: Cloning and sequencing of an Agrobacterium tumefaciens β-glucosidase gene involved in modifying a vir-inducing plant signal molecule. J Bact 174: 1478–1486 (1992).

    Google Scholar 

  7. Chen C-Y, Winans SC: Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter. J Bact 173: 1139–1144 (1991).

    Google Scholar 

  8. Chen C-Y, Winans SC: Characterization of the supervirulent virG gene of the Agrobacterium tumefaciens plasmid pTiBo542. Mol Gen Genet 230: 302–309 (1991).

    Google Scholar 

  9. Chyi Y-S, Jorgensen RA, Goldstein D, Tanksley SD, Loaiza-Figueroa F: Locations and stability of Agrobacterium-mediated T-DNA insertions in the Lycopersicon genome. Mol Gen Genet 204: 64–69 (1986).

    Google Scholar 

  10. Czakó M, An G: Expression of DNA coding for diphteria toxin chain A is toxic to plant cells. Plant Physiol 95: 687–692 (1991).

    Google Scholar 

  11. Dean C, Sjodin C, Page T, Jones J, Lister C: Behaviour of the maize transposable element Ac in Arabidopsis thaliana. Plant J 2: 69–81 (1992).

    Google Scholar 

  12. Depicker A, Jacobs AM, VanMontagu M: A negative selection scheme for tobacco protoplast-derived cells expressing the T-DNA gene 2. Plant Cell Rep 7: 63–66 (1988).

    Google Scholar 

  13. Dooner HK, Keller J, Harper E, Ralston E: Variable patterns of transposition of the maize element Activator in tobacco. Plant Cell 3: 473–482 (1991).

    Google Scholar 

  14. Errampali D, Patton D, Castle L, Mickelson L, Hansen K, Schnall J, Feldmann K, Meinke D: Embryogenic lethals and T-DNA insertional mutagenesis in Arabidopsis. Plant Cell 3: 149–157 (1991).

    Google Scholar 

  15. Estelle MA, Somerville CR: The mutants of Arabidopsis. Trends Genet 2: 92–96 (1986).

    Google Scholar 

  16. Estruch JJ, Prinsen E, VanOnckelen H, Schell J, Spena A: Viviparous leaves produced by somatic activation of an inactive cytokinin synthesizing gene. Science 254: 1364–1367 (1991).

    Google Scholar 

  17. Feldmann KA: T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum. Plant J 1: 71–82 (1991).

    Google Scholar 

  18. Feldmann KA: T-DNA insertion mutagenesis in Arabidopsis: seed infection/transformation. In: Koncz C, Chua N-H, Schell J (eds) Methods in Arabidopsis Research, pp. 274–289. World Scientific, Singapore (1992).

    Google Scholar 

  19. Feldmann KA, Marks MD, Christianson ML, Quatrano RS: A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science 243: 1351–1354 (1989).

    Google Scholar 

  20. Fobert PR, Miki BL, Iyer VN: Detection of gene regulatory signals in plants revealed by T-DNA-mediated fusions. Plant Mol Biol 17: 837–851 (1991).

    Google Scholar 

  21. Galbraith DW, Harkins KR, Knapp S: Systemic endoploiploidy in Arabidopsis thaliana. Plant Physiol 96: 985–989 (1991).

    Google Scholar 

  22. Gerlitz M, Hrabak O, Schwab H: Partitioning of broadhost-range plasmid RP4 is a complex system. J Bact 172: 6194–6203 (1990).

    Google Scholar 

  23. Gheysen G, Villarroel R, VanMontagu M: Illegitimate recombination in plants: a model for T-DNA integration. Genes Devel 5: 287–297 (1991).

    Google Scholar 

  24. Goldsbrough A, Bevan M: New patterns of gene activity in plants detected using an Agrobacterium vector. Plant Mol Biol 16: 263–269 (1991).

    Google Scholar 

  25. Gould J, Devey M, Hasegawa O, Ulian EC, Peterson G, Smith RM: Transformation of Zea mays L. using Agrobacterium tumefaciens and the shoot apex. Plant Physiol 95: 426–434 (1990).

    Google Scholar 

  26. Grandbastien M-A: Retroelements in higher plants. Trends Genet 8: 103–108 (1992).

    Google Scholar 

  27. Grant V: Genetics of Flowering Plants. Columbia University Press, New York (1975).

    Google Scholar 

  28. Grevelding C, Becker D, Kunze R, Von Menges A, Fantes V, Schell J, Masterson R: High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis. Proc Natl Acad Sci USA 89, in press (1992).

  29. Halfter U, Morris PC, Willmitzer L: Gene targeting in Arabidopsis thaliana. Mol Gen Genet 231: 186–193 (1991).

    Google Scholar 

  30. Haring MA, Rommens CMT, Nijkamp HJJ, Hille J: The use of transgenic plants to understand transposition mechanism and to develop transposon tagging strategies. Plant Mol Biol 16: 449–461 (1991).

    Google Scholar 

  31. Herman PL, Marks MD: Trichome development in Arabidopsis thaliana. II. Isolation and complementation of the Glabrous 1 gene. Plant Cell 1: 1051–1055 (1989).

    Google Scholar 

  32. Herman L, Jacobs A, VanMontagu M, Depicker A: Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events. Mol Gen Genet 224: 248–256 (1990).

    Google Scholar 

  33. Herrera-Estrella A, VanMontagu M, Wang K: A bacterial peptide acting as plant nuclear targeting signal: the amino-terminal portion of Agrobacterium VirD2 protein directs a β-galactosidase fusion protein into tobacco nuclei. Proc Natl Acad Sci USA 87: 9534–9537 (1990).

    Google Scholar 

  34. Hess KM, Dudley MW, Lynn DG, Joerger RD, Binns AN: Mechanism of phenolic activation of Agrobacterium virulence genes: Development of a specific inhibitor of bacterial sensor/response system. Proc Natl Acad Sci USA 88: 7854–7858 (1991).

    Google Scholar 

  35. Hooykaas PJJ, Schilperoort RA: Agrobacterium and plant genetic engineering. Plant Mol Biol 19: 15–38 (1992).

    Google Scholar 

  36. Howard E, Zupan JR, Citovsky V, Zambryski PC: The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: Implications for nuclear uptake of DNA in plant cells. Cell 68: 109–118 (1992).

    Google Scholar 

  37. Huijser P, Klein J, Lönnig W-E, Meijer H, Saedler H, Sommer H: Bracteomania, an inflorescence anomaly, is caused by the loss of function of the MADS-box gene squamosa in Antirrhinum majus. EMBO J 11: 1239–1249 (1992).

    Google Scholar 

  38. Hwang I, Kohchi T, Hauge BM, Goodman HM, Schmidt R, Chops G, Dean C, Gibson S, Iba K, Lemieux B, Arondel V, Danhoff L, Somerville C: Identification and map position of YAC clones comprising one-third of the Arabidopsis genome. Plant J 1: 367–374 (1991).

    Google Scholar 

  39. Jack T, Brockman LL, Meyerowitz EM: The homeotic gene APETALA 3 of Arabidopsis thaliana encodes a MADS-box and is expressed in petals and stamens. Cell 68: 683–697 (1992).

    Google Scholar 

  40. Jürgens G, Mayer U, Torres Ruiz RA, Berleth T, Miséra S: Genetic analysis of pattern formation in the Arabidopsis embryo. Development (suppl.) 1: 27–38 (1991).

    Google Scholar 

  41. Karlin-Neuman GA, Brusslan JA, Tobin EM: Phytochrome control of the tms2 gene in transgenic Arabidopsis: A strategy for selecting mutants in the signal transduction pathway. Plant Cell 3: 573–582 (1991).

    Google Scholar 

  42. Keller J, Lim E, James DW, Dooner HK: Germinal and somatic activity of the maize element activator (Ac) in Arabidopsis. Genetics 131: 449–459 (1992).

    Google Scholar 

  43. Kertbundit S, DeGreve H, Deboeck F, VanMontagu M, Hernalsteens J-P: In vivo random β-glucuronidase gene fusions in Arabidopsis thaliana. Proc Natl Acad Sci USA 88: 5212–5216 (1991).

    Google Scholar 

  44. Koncz C, Kreutzaler F, Kalman Z, Schell J: A simple method to transfer, integrate and study expression of foreign genes, such as chicken ovalbumin and α-actin in plant tumours. EMBO J 3: 1929–1937 (1984).

    Google Scholar 

  45. Koncz C, Martini N, Koncz-Kalman Z, Ollson O, Radermacher A, Szalay AA, Schell J: Genetic tools for the analysis of gene expression in plants. In Bruening G, Harada J, Kosuge T, Hollaender A (eds) Tailoring Genes for Crop Impovement, pp. 197–209. Plenum Press, New York (1987).

    Google Scholar 

  46. Koncz C, Martini N, Mayerhofer R, Koncz-Kalman Z, Körber H, Rédei GP, Schell J: High-frequency T-DNA-mediated gene tagging in plants. Proc Natl Acad Sci USA 86: 8467–8471 (1989).

    Google Scholar 

  47. Koncz C, Langridge WHR, Olsson O, Schell J, Szalay AA: Bacterial and firefly luciferase genes in transgenic plants: Advantages and disadvantagess of a reporter gene. Devel Genet 11: 224–232 (1990).

    Google Scholar 

  48. Koncz C, Mayerhofer R, Koncz-Kalman Z, Nawrath C, Reiss B, Rédei GP, Schell J: Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana. EMBO J 9: 1337–1346 (1990).

    Google Scholar 

  49. Koncz C, Chua N-H, Schell J: Methods in Arabidopsis Research. World Scientific, Singapore (1992).

    Google Scholar 

  50. Koncz C, Schell J, Rédei GP: T-DNA transformation and insertion mutagenesis. In: Koncz C, Chua N-H, Schell J (eds) Methods in Arabidopsis Research. pp. 224–273. World Scientific, Singapore (1992).

    Google Scholar 

  51. Koning A, Jones A, Fillatti JJ, Comai L, Lassner MW: Arrest of embryo development in Brassica napus mediated by modified Pseudomonas aeruginosa exotoxin A. Plant Mol Biol 18: 247–248 (1992).

    Google Scholar 

  52. Körber H, Strizhov N, Staiger D, Feldwisch J, Olsson O, Sandberg G, Palme K, Schell J, Koncz C: T-DNA gene 5 of Agrobacterium modulates auxin response by autoregulated synthesis of a growth hormone antagonist in plants. EMBO J 10: 3983–3991 (1991).

    Google Scholar 

  53. Langridge WHR, Fitzgerald KJ, Koncz C, Schell J, Szalay AA: Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones. Proc Natl Acad Sci USA 86: 3219–3223 (1989).

    Google Scholar 

  54. Lee KY, Lund P, Lowe K, Dunsmuir P: Homologous recombination in plant cells after Agrobacterium-mediated transformation. Plant Cell 2: 415–425 (1990).

    Google Scholar 

  55. Ma H, Yanofski MF, Meyerowitz EM: AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. Genes Devel 5: 484–495 (1991).

    Google Scholar 

  56. Mariani C, DeBeuckeleer M, Truettner J, Leemans J, Goldberg RB: Induction of male-sterility in plants by a chimeric ribonuclease gene. Nature 347: 737–741 (1990).

    Google Scholar 

  57. Marks MD, Feldmann KA: Trichome development in Arabidopsis thaliana. I. T-DNA tagging of the Glabrous 1 gene. Plant Cell 1: 1043–1050 (1989).

    Google Scholar 

  58. Matsumoto S, Ito Y, Hosoi T, Takahashi Y, Machida Y: Integration of Agrobacterium T-DNA into a tobacco chromosome: possible involvement of DNA homology between T-DNA and plant DNA. Mol Gen Genet 224: 309–316 (1990).

    Google Scholar 

  59. Mayerhofer R, Koncz-Kalman Z, Nawrath C, Bakkeren G, Crameri A, Angelis K, Rédei GP, Schell J, Hohn B, Koncz C: T-DNA integration: a mode of illegitimate recombination in plants. EMBO J 10: 697–704 (1991).

    Google Scholar 

  60. Meyerowitz EM: Arabidopsis thaliana. Annu Rev Genet 21: 93–111 (1987).

    Google Scholar 

  61. Meyerowitz EM, Pruitt RE: Arabidopsis thaliana and plant molecular genetics. Science 229: 1214–1218 (1985).

    Google Scholar 

  62. Murai N, Li Z, Kawagoe Y, Hayashimoto A: Transposition of maize activator element in transgenic rice plants. Nucl Acids Res 19: 617–622 (1991).

    Google Scholar 

  63. Oppenheimer DG, Herman PL, Sivakumaran S, Esch J, Marks DM: A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell 67: 483–493 (1991).

    Google Scholar 

  64. Orr JD, Lynn DG: Biosynthesis of dehydrodiconiferyl alcohol glucosides: Implications for the control of tobacco cell growth. Plant Physiol 98: 343–352 (1991).

    Google Scholar 

  65. Osborne BI, Corr CA, Prince JP, Hehl R, Tanksley SD, McCormick S, Baker B: Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome. Genetics 129: 833–844 (1991).

    Google Scholar 

  66. Pazour GJ, Ta CN, Das A: Mutants of Agrobacterium tumefaciens with elevated vir gene expression. Proc Natl Acad Sci USA 88: 6941–6945 (1991).

    Google Scholar 

  67. Peleman J, Cottyn B, VanCamp W, VanMontagu M, Inzé D: Transient occurrence of extrachromosomal DNA of an Arabidopsis thaliana transposon-like element, Tatl. Proc Natl Acad Sci USA 88: 3618–3622 (1991).

    Google Scholar 

  68. Pnueli L, Abu-Abeid M, Zamir D, Nacken W, Schwarz-Sommer Z, Lifschitz E: The MADS-box gene family in tomato: temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis. Plant J 1: 255–266 (1992).

    Google Scholar 

  69. Prior TI, FitzGerald DJ, Pastah I: Barnase toxin: A new chimeric toxin composed of Pseudomonas exotoxin A and barnase. Cell 64: 1017–1023 (1991).

    Google Scholar 

  70. Rédei GP: Arabidopsis as a genetic tool. Annu Rev Genet 9: 11–151 (1975).

    Google Scholar 

  71. Rédei GP: Genetics. Macmillan, New York (1982).

    Google Scholar 

  72. Rédei GP, Koncz C, Schell J: Transgenic Arabidopsis. In: Gustafson JP, Appels R (eds) Chromosome Structure and Function, pp 175–200. Plenum, New York (1988).

    Google Scholar 

  73. Roberts RC, Burioni R, Helinski DR: Genetic characterization of the stabilizing functions of a region of broadhost-range plasmid RK2. J Bact 172: 6204–6216 (1990).

    Google Scholar 

  74. Schläppi M, Hohn B: Competence of immature maize embryos for Agrobacterium-mediated gene transfer. Plant Cell 4: 7–16 (1992).

    Google Scholar 

  75. Schmidt R, Willmitzer L: The maize autonomous element Activator (Ac) shows a minimal germinal excision frequency of 0.2–0.5% in transgenic Arabidopsis thaliana plants. Mol Gen Genet 220: 17–24 (1989).

    Google Scholar 

  76. Schwarz-Sommer Z, Huijser P, Nacken W, Saedler H, Sommer H: Genetic control of flower development by homeotic genes in Antirrhinum majus. Science 250: 931–936 (1990).

    Google Scholar 

  77. Schwarz-Sommer Z, Hue I, Huijser P, Flor JP, Hansen R, Tetens F, Lönnig W-E, Saedler H, Sommer H: Characterization of the Antirrhinum floral homeotic MADS-box gene deficiens: evidence for DNA binding and autoregulation of its persistent expression throughout flower development. EMBO J 11: 251–263 (1992).

    Google Scholar 

  78. Swinburne J, Balcells L, Scofield SR, Jones JDG, Coupland G: Elevated levels of Activator transposase mRNA are associated with high frequencies of Dissociation excision in Arabidopsis. Plant Cell 4: 583–595 (1992).

    Google Scholar 

  79. Teeri TH, Herrera-Estrella L, Depicker A, VanMontagu M, Palva ET: Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the nptII gene. EMBO J 5: 1755–1760 (1986).

    Google Scholar 

  80. Thorsness MK, Kandasamy MK, Nasrallah ME, Nasrallah JB: Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana. Devel Biol 143: 173–184 (1991).

    Google Scholar 

  81. Topping JF, Wei W, Lindsey K: Functional tagging of regulatory elements in the plant genome. Development 112: 1009–1019 (1991).

    Google Scholar 

  82. Towar J, Lichtenstein C: Somatic and meiotic chromosomal recombination between inverted duplications in transgenic tobacco plants. Plant Cell 4: 319–332 (1992).

    Google Scholar 

  83. Turk SCHJ, Melchers LS, denDulk-Ras H, Regensburg-Tuink AJG, Hooykaas PJJ: Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Plant Mol Biol 16: 1051–1059 (1991).

    Google Scholar 

  84. Vancanneyt G, Schmidt R, O'Connor-Sanchez A, Willmitzer L, Rosa-Sosa M: Construction of an introncontaining marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220: 245–250 (1990).

    Google Scholar 

  85. VanLijsebettens M, Vanderhaegen R, VanMontagu M: Insertional mutagenesis in Arabidopsis thaliana: isolation of a T-DNA-linked mutation that alters leaf morphology. Theor Appl Genet 81: 277–284 (1991).

    Google Scholar 

  86. Voytas DF, Konieczny A, Cummings HP, Ausubel FM: The structure, distribution and evolution of the Ta1 retrotransposable element family of Arabidopsis thaliana. Genetics 126: 713–721 (1990).

    Google Scholar 

  87. Walden R, Koncz C, Schell J: The use of gene vectors in plant molecular biology. Meth Mol Cell Biol 4: 175–194 (1990).

    Google Scholar 

  88. Walden R, Hayashi H, Schell J: T-DNA as a gene tag. Plant J 1: 281–288 (1991).

    Google Scholar 

  89. Wallroth M, Gerats AGM, Rogers SG, Fraley RT, Horsch RB: Chromosomal localization of foreign genes in Petunia hybrida. Mol Gen Genet 202: 6–15 (1986).

    Google Scholar 

  90. Walker C, Weinstein JD: In vitro assay of the chlorophyll biosynthetic enzyme Mg-chelatase: Resolution of the activity into soluble and membrane-bound fractions. Proc Natl Acad Sci USA 88: 5789–5793 (1991).

    Google Scholar 

  91. Yanofski MF, Ma H, Bowman JL, Drews GN, Feldmann KA, Meyerowitz EM: The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature 346: 35–38 (1990).

    Google Scholar 

  92. Zambryski P, Tempé J, Schell J: Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants. Cell 56: 193–201 (1989).

    Google Scholar 

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  1. Max-Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-5000, Köln 30, Germany

    Csaba Koncz, Kinga Németh & Jeff Schell

  2. Institute of Plant Physiology, Biological Research Center of Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    Csaba Koncz & Kinga Németh

  3. 3005 Woodbine Ct., 65203-0906, Columbia, MO, USA

    George P. Rédei

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Koncz, C., Németh, K., Rédei, G.P. et al. T-DNA insertional mutagenesis in Arabidopsis . Plant Mol Biol 20, 963–976 (1992). https://doi.org/10.1007/BF00027166

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