Recent Advances in Virus Diagnosis

1. Front Matter

   Pages I-VIII

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2. Viral Diagnosis by Immunofluorescence

   • P. S. Gardner

   Pages 1-14

3. Applications of Immunofluorescence in Veterinary Viral Diagnosis

   • M. S. McNulty, G. M. Allan

   Pages 15-26

4. Tagging of Viral Antigens using Immunoperoxidase and Immunogold Techniques

   • R. Ducatelle, F. Castryck, J. Hoorens

   Pages 27-36

5. Detection of BVD Virus in Viremic Cattle by an Indirect Immunoperoxidase Technique

   • A. Meyling

   Pages 37-46

6. Viral Diagnosis by Electron Microscopy

   • June D. Almeida

   Pages 47-59

7. Capture Assays for the Detection of Virus-Specific IgM Antibody

   • Richard S. Tedder

   Pages 60-71

8. Detection and Identification of Papillomaviruses in Benign and Malignant Tumours of Cattle

   • M. Saveria Campo

   Pages 72-94

9. Detection of Viral Nucleic Acids in Cell Cultures and in Clinical Specimens by Spot Hybridization

   • T. Hyypia, P. Stalhandske, R. Vainionpaa, P. Halonen, U. Pettersson

   Pages 95-100

10. Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA

    • A. L. J. Gielkens, J. T. van Oirschot, F. W. van Schie, B. Toma

    Pages 101-114

11. Protein Blotting: The Basic Method and its Role in Viral Diagnosis

    • A. J. Herring, J. M. Sharp

    Pages 115-124

12. The Use of Polyacrylamide Gel Electrophoresis of Virus RNA in the Study of Rotavirus Infections

    • D. Todd, M. S. McNulty, G. M. Allan

    Pages 125-135

13. Examination of Rotavirus from Bovine and Porcine Species by Western Blotting

    • Anette G. Boetner, Jon Askaa

    Pages 136-144

14. Use of Monoclonal Antibodies in Virus Diagnosis

    • D. van Zaane

    Pages 145-156

15. Bovine Leukosis — Recent Developments with use of Monoclonal Antibodies and Elisa Tests

    • D. Portetelle, C. Bruck, M. Mammerickx, A. Burny

    Pages 157-170

16. Radioimmunoassay

    • Daniel Levy

    Pages 171-185

17. Immunoelectrophoretic Techniques

    • K. Dalsgaard

    Pages 186-194

18. Diagnostic use of CNS Antibodies

    • Kurt Danner

    Pages 195-210

19. Back Matter

    Pages 211-213

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The traditional approach to diagnosis of virus infectians by isolation of the causative virus is usually both slow and expensive. More recently, the emphasis has been on the direct detection of viruses or viral antigens in clinical specimens. This can be done using established techniques such as immunofluorescence or electron microscopy, or by newly developed biochemical methods. The purpose of this meeting was to review these and other developments in the laboratory diagnosis of virus infections. We would like to thank all those who contributed to the success of this meeting. In particular, we are grateful to the CEC for financial sponsorship, to Professor C. Dow, Director of the Veterinary Research Laboratories, for help in organising the meeting, and to Miss B. Hamilton for her excellent typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS Page Preface V VIRAL DIAGNOSIS BY TIMMUNOFLUORESCENCE P. S. Gardner APPLICATIONS OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL DIAGNOSIS M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS USING IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC CATTLE BY AN INDIRECT IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL DIAGNOSIS BY ELECTRON MICROSCOPY J. D. Almeida 47 CAPTURE ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND IDENTIFICATION OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF CATTLE M. S. Campo 72 DETECTION OF VIRAL NUCLEIC ACIDS IN CELL CULTURES .

• animal pathology
• antibody
• antigen
• cell
• cell culture
• diagnosis
• infection
• pathology
• virus

• Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland

  M. S. McNulty, J. B. McFerran

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