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Molecular Tools for Screening Biodiversity

Plants and Animals

  • Book
  • © 1998

Overview

Editors:
  1. Angela Karp

    1. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, UK

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  2. Peter G. Isaac

    1. Agrogene SA, Moissy Cramayel, France

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    You can also search for this editor in PubMed   Google Scholar

  3. David S. Ingram

    1. Regius Keeper of the Royal Botanic Garden Edinburgh, UK

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Table of contents (83 chapters)

  1. Front Matter

    Pages i-xxiv
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  2. DNA Extraction

    1. Front Matter

      Pages 1-1
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    2. DNA Extraction: widely applicable methods

      1. Isolation of Animal Cellular Total DNA
        • De-Xing Zhang, Godfrey M. Hewitt
        Pages 5-9
      2. Plant Total DNA Extraction
        • Julia Rueda, Rosario Linacero, Ana M. Vázquez
        Pages 10-14
      3. Chloroplast DNA Extraction Procedure for Species High in Phenolics and Polysaccharides
        • Roselyne Lumaret, Henri Michaud, Jean-Philippe Ripoll, Lamjed Toumi
        Pages 15-17
      4. Quantification of DNA
        • Rosario Linacero, Julia Rueda, Ana M. Vázquez
        Pages 18-21
      5. Miniprep Procedures for the Isolation of Plant DNA
        • Keith J. Edwards
        Pages 22-24

    3. DNA Extraction: methods for difficult species/tissues

      1. Special DNA Extraction Methods for Some Animal Species
        • De-Xing Zhang, Godfrey M. Hewitt
        Pages 27-31
      2. Methods for Difficult Plant Species/Tissues
        • Birgit Ziegenhagen, Florian Scholz
        Pages 32-35
      3. DNA Maxipreparations: A Comparison of Protocols for Rapid Extraction of Good Quality DNA from Recalcitrant Species
        • Birgit Ziegenhagen, Florian Scholz
        Pages 36-37

    4. Preserved specimens

      1. Isolation of DNA from Preserved Specimens
        • De-Xing Zhang, Godfrey M. Hewitt
        Pages 41-45
      2. Field Collection: Animals
        • De-Xing Zhang, Godfrey M. Hewitt
        Pages 46-48
      3. Field Collection: Plants
        • Roger Hyam
        Pages 49-50

    5. DNA Extraction using anion-exchange chromatography and silica-gel based membranes

      1. DNA Extraction Using Anion-Exchange Chromatography and Silica-Gel Based Membranes
        • Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian
        Pages 53-54
      2. Isolation of Nucleic Acids Using Anion-Exchange Chromatography: QIAGEN-tip Based Methods
        • Angela Karp, Peter G. Isaac, David S. Ingram
        Pages 54-59
      3. Isolation of Nucleic Acids Using Silica-Gel Based Membranes: Methods Based on the Use of QIAamp Spin Columns
        • Angela Karp, Peter G. Isaac, David S. Ingram
        Pages 59-63
      4. Isolation of Total RNA from Animal Tissue Using Anion-Exchange Chromatography (QIAGEN-tips)
        • Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian
        Pages 64-66
      5. Isolation of Total RNA Using Silica-Gel Based Membranes
        • Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian
        Pages 67-70

  3. Basic Screening Methods

    1. Front Matter

      Pages 71-71
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    2. Isozymes

      1. Isozymes
        • Gerhard Müller-Starck
        Pages 75-81
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Keywords

About this book

Mark Chase There are many literature resources available to molecular biologists wishing to assess genetic variation, but the myriad of techniques and approaches potentially available to the plant breeder and the evolutionary biologist is truly bewildering, and most have never been evaluated side-by-side on the same sets of samples. Additionally, it is often not recognized that tools that are useful for breeders can often be adapted for use in evolutionary studies and vice versa, but this is generally the case. The borderline between population genetics and phylogenetics is vague and difficult to assess, and a combination of both types of tools is best when it is not clear with which area one is dealing. Furthermore, it is not now appropriate to use just one type of marker in any kind of study; most markers have the potential to misinform under certain conditions, so it is always wise to incorporate at least two different types of assessments into any project. This volume is designed to facilitate this sort of multiple approach and provides comparative data on most currently available methods so that researchers can more intelligently select those appropriate to their area of interest, regardless of whether it is in the realm of breeding or evolutionary biology.

Editors and Affiliations

  • IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, UK

    Angela Karp

  • Agrogene SA, Moissy Cramayel, France

    Peter G. Isaac

  • Regius Keeper of the Royal Botanic Garden Edinburgh, UK

    David S. Ingram

Bibliographic Information

  • Book Title: Molecular Tools for Screening Biodiversity

  • Book Subtitle: Plants and Animals

  • Editors: Angela Karp, Peter G. Isaac, David S. Ingram

  • DOI: https://doi.org/10.1007/978-94-009-0019-6

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: Chapman & Hall 1998

  • Hardcover ISBN: 978-0-412-63830-5Published: 31 December 1997

  • Softcover ISBN: 978-94-010-6496-5Published: 09 November 2011

  • eBook ISBN: 978-94-009-0019-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 528

  • Topics: Nature Conservation, Animal Anatomy / Morphology / Histology, Evolutionary Biology

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