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Practical Plant Virology

Protocols and Exercises

  • Book
  • © 1998

Overview

Authors:
  1. Jeanne Dijkstra

    1. Department of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

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    You can also search for this author in PubMed   Google Scholar

  2. Cees P. Jager

    1. Department of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

    View author publications

    You can also search for this author in PubMed   Google Scholar

  • Detailed protocols for easy use in the laboratory
  • First manual covering the whole field of plant virology
  • Both authors have been involved in virology teaching and research for over 30 years
  • With special exercises for teaching purposes

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (65 chapters)

  1. Front Matter

    Pages I-XVI
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  2. Virus Inoculation

    1. Front Matter

      Pages 1-4
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    2. Mechanical Inoculation of Plants

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 5-13

    3. Inoculation of Protoplasts Isolated from Leaves

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 14-28

    4. Differential Temperature Treatment of Infected Plants

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 29-31

    5. Infectivity of Tobacco Mosaic Tobamovirus

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 32-33

  3. Symptomatology

    1. Front Matter

      Pages 35-41
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    2. Description of External Symptoms

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 42-47

    3. Determination of Internal Symptoms

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 48-56

    4. Systemic Acquired Resistance (SAR)

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 57-59

    5. Demonstration of Phloem Necrosis in Plants Affected by Potato Leafroll

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 60-62

    6. Inclusions in Hair Cells of Leaves of Tobacco Infected with Tobacco Mosaic Tobamovirus

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 63-64

  4. Infectivity Assays

    1. Front Matter

      Pages 65-65
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    2. Infectivity Assay on Local-Lesion Hosts

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 67-77

    3. Infectivity Assay on Hosts Producing Starch Lesions

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 78-81

    4. Infectivity Assay on Hosts Producing Systemic Symptoms Only

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 82-85

    5. Effect of Temperature on the Susceptibility of Plants

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 86-87

    6. Effect of Abrasives on the Infection

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 88-89

    7. Effect of Inhibitors on the Infection

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 90-91

    8. Effect of Light on the Susceptibility of Plants

      • Jeanne Dijkstra, Cees P. de Jager
      Pages 92-93
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Keywords

About this book

The idea for this book arose from what we perceived as the need for an up-to-date guide to class exercises in plant virology. We were encouraged to proceed after receiving 29 positive responses (out of 30 replies to our enquiries) from colleagues worldwide. To the best of our knowledge, no such publications have appeared since D. Noordam's book containing practical exercises (Noordam 1973) and the latest (1988) edition of the American Phytopathological Society's Laboratory Exercises in Plant Pathology, in which 4 out of its 31 chapters discuss plant viruses. Our original plan was to aim this publication at students and teachers of plant virology, plant pathology, plant breeding and microbiology. How­ ever, both colleagues and our publisher suggested widening the scope of the book by making it useful also for research workers and laboratory technicians. Therefore, we decided to prepare a laboratory manual of interest to all groups. We have tried to cover all relevant branches of plant virology, including the molecular aspects, in as far as they pertain to the detection and basic characterisation of plant viruses. We have not included protocols for the molecular biology of plant viruses (sequencing, construction of recombi­ nants, transgenic plants, etc.), as they are presented adequately in many other recent publications. The protocols in this book are described in a manner which should be understandable to those with a basic knowledge of biology and chemistry.

Authors and Affiliations

  • Department of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

    Jeanne Dijkstra, Cees P. Jager

Bibliographic Information

  • Book Title: Practical Plant Virology

  • Book Subtitle: Protocols and Exercises

  • Authors: Jeanne Dijkstra, Cees P. Jager

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-72030-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • eBook ISBN: 978-3-642-72030-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XVI, 459

  • Number of Illustrations: 152 b/w illustrations, 21 illustrations in colour

  • Topics: Plant Sciences, Agriculture, Forestry, Microbiology

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