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Single-Molecule Metal-Induced Energy Transfer

From Basics to Applications

  • Book
  • © 2017

Overview

Authors:
  1. Narain Karedla

    1. Third Institute of Physics—Biophysics, Göttingen University, Göttingen, Germany

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  • Nominated as an outstanding PhD thesis by Göttingen University, Germany
  • Introduces the electrodynamics of a dipole emitter from fundamentals with illustrations
  • Includes descriptions of the optical setups used, together with possible modifications/combinations that will be especially useful for experimental physicists
  • Features discussion/comments on the practical aspects of the method, highlighting its potential applications in biophysics, structural biology, and molecular physics
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Theses (Springer Theses)

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Table of contents (6 chapters)

  1. Front Matter

    Pages i-xviii
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  2. Introduction

    • Narain Karedla
    Pages 1-16

  3. Theory

    • Narain Karedla
    Pages 17-74

  4. Single-Molecule Metal-Induced Energy Transfer (smMIET)

    • Narain Karedla
    Pages 75-85

  5. Single-Molecule Transition Dipole Imaging

    • Narain Karedla
    Pages 87-143

  6. Discussion and Outlook

    • Narain Karedla
    Pages 145-158

  7. Conclusion

    • Narain Karedla
    Pages 159-160

  8. Back Matter

    Pages 161-166
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Keywords

About this book

This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.

Authors and Affiliations

  • Third Institute of Physics—Biophysics, Göttingen University, Göttingen, Germany

    Narain Karedla

About the author

Narain Karedla is an experimental physicist at the Third Institute of Physics-Biophysics at Göttingen University, Germany. Narain defended his doctoral research on 'Single-molecule Metal Induced Energy Transfer (smMIET): From Basics to Applications' in June 2016 with the highest distinction (summa cum laude). His current scientific interests include developing single-molecule spectroscopy and superresolution techniques for  biophysical applications. He holds an integrated Master of Science degree in Chemistry from the Indian Institute of Technology, Roorkee.

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