Next Generation Sequencing in Cancer Research, Volume 2

1. Front Matter

   Pages i-xviii

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2. Single-Cell Next-Generation Sequencing and Its Applications in Cancer Biology

   • Biaoru Li, Xiaomeng Zhang, Jie Zheng

   Pages 1-18

3. Utility of Next-Generation Sequencing in Cancer Drug Development and Clinical Trials

   • François Thomas, Ahmad Awada

   Pages 19-37

4. Next-Generation Sequencing in the Era of Cancer-Targeted Therapies: Towards the Personalised Medicine

   • Ashwag Albukhari, Fawzi F. Bokhari, Hani Choudhry

   Pages 39-55

5. Mutational Similarities Across Cancers: Implications for Research, Diagnostics, and Personalized Therapy Design

   • Frederick Klauschen, Albrecht Stenzinger, Daniel Heim

   Pages 57-65

6. Standardized Decision Support in NGS Reports of Somatic Cancer Variants

   • Rodrigo Dienstmann

   Pages 67-80

7. Clinical Considerations in the Conduct of Cancer Next-Generation Sequencing Testing and Genetic Counseling

   • Heather Fecteau, Tuya Pal

   Pages 81-101

8. Next-Generation Sequencing for Cancer Biomarker Discovery

   • Aarti N. Desai, Abhay Jere

   Pages 103-125

9. Validation and Implementation of Next-Generation Sequencing Technologies in a Clinical Molecular Diagnostic Laboratory

   • Rajesh R. Singh, Rajyalakshmi Luthra

   Pages 127-135

10. Next-Generation Sequencing Technologies and Formalin-Fixed Paraffin-Embedded Tissue: Application to Clinical Cancer Research

    • Nadine Norton

    Pages 137-154

11. Applications of NGS to Screen FFPE Tumours for Detecting Fusion Transcripts

    • Kunbin Qu, Joffre Baker, Yan Ma

    Pages 155-177

12. Clinical Applications of Next-Generation Sequencing of Formalin-Fixed Paraffin-Embedded Tumors

    • Cheryl L. Thompson, Vinay Varadan

    Pages 179-192

13. ChIP-BS-Sequencing in Cancer Epigenomics

    • Karthikraj Natarajan, Fei Gao

    Pages 193-210

14. Integrative Analysis Identifies Transcription Factor–DNA Methylation Relationships and Introduces New Avenues for Translating Cancer Epigenetics into the Clinic

    • Matthew H. Ung, Shaoke Lou, Frederick S. Varn, Chao Cheng

    Pages 211-228

15. Differential Methylation Analysis with Next-Generation Sequencing

    • Hongyan Xu

    Pages 229-238

16. Performance Comparison and Data Analysis Strategies for MicroRNA Profiling in Cancer Research

    • Erik Knutsen, Maria Perander, Tonje Fiskaa, Steinar D. Johansen

    Pages 239-265

17. Small RNA Sequencing for Squamous Cell Carcinoma Research

    • Patricia Severino, Liliane Santana Oliveira, Alan Mitchell Durham

    Pages 267-277

18. Exome Capture and Capturing Technologies in Cancer Research

    • Chandra Sekhar Reddy Chilamakuri, Leonardo A. Meza-Zepeda

    Pages 279-302

19. The Landscape of DNA Virus Associations Across Human Cancers

    • Jian Chen, Lopa Mishra, Xiaoping Su

    Pages 303-315

20. Using Next-Generation Sequencing to Reveal Patterns of Chromosomal Alterations in Oral Verrucous Lesions

    • Manar Samman, Neeraj Sethi

    Pages 317-343

Latest generation sequencing revolutionizes the fields of cancer research and oncology. This follow-up volume focuses more extensively on single cell sequencing of cancer and trials in drug resistance. Another exciting feature is the bioinformatics tools given, that can be used on cancer genome studies. Scientists around the world are attempting to find the root cause of cancer. A reasonable cancer treatment plan and potential cure is more optimistic now with the unfolding of the cancer genome. The collective knowledge of how to leverage next generation sequencing in cancer research is paving the way. The important information provided in this volume will move the field forward in developing novel targeted cancer therapies.

• RNAs
• Whole-genome sequencing
• cancer genomics
• high-throughput sequencing
• transcriptome

• Department of Pathology and Laboratory, Medicine, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Canada

  Wei Wu

• Faculty of Science, Biochemistry Department, Center of Innovation in Personalized Medicine, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, Saudi Arabia

  Hani Choudhry

Wei Wu, M.D., Ph.D. is a Research Associate for the Department of Bio Science at the University of Calgary, specializing in the cancer genome and systems biology. He has edited two volumes with Springer. In addition, he's a member of various editorial board for journals including the International Journal of Biomedical Sciences, International Journal of Molecular Medicine and Advances in Sciences and the Research Journal of Biological Sciences. Hani Choudhry, D.Phil is a member of the Genomics Research Group at the Wellcome Trust Centre for Human Genetics at Oxford University. His expertise is in high throughput next generation sequencing.

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