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Plant Transposable Elements

Methods and Protocols

Editors:

  • Details background information and specific experimental protocols on plant transposable elements
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1057)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
  2. Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity

    • Lakshminarasimhan Krishnaswamy, Thomas Peterson
    Pages 43-58
  3. Molecular Biology of Maize Ac/Ds Elements: An Overview

    • Katina Lazarow, My-Linh Doll, Reinhard Kunze
    Pages 59-82
  4. Gene Tagging with Engineered Ds Elements in Maize

    • Yubin Li, Gregorio Segal, Qinghua Wang, Hugo K. Dooner
    Pages 83-99
  5. Plant Regeneration Methods for Rapid Generation of a Large Scale Ds Transposant Population in Rice

    • Yuan Hu Xuan, Jin Huang, Gihwan Yi, Dong-Soo Park, Soo Kwon Park, Moo Young Eun et al.
    Pages 101-116
  6. Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting

    • Dafang Wang, Thomas Peterson
    Pages 117-122
  7. Using MuDR/Mu Transposons in Directed Tagging Strategies

    • Virginia Walbot, Julia Qüesta
    Pages 143-155
  8. Genetic and Molecular Analyses of UniformMu Transposon Insertion Lines

    • Donald R. McCarty, Masaharu Suzuki, Charles Hunter, Joseph Collins, Wayne T. Avigne, Karen E. Koch
    Pages 157-166
  9. Reverse Genetics in Rice Using Tos17

    • Delphine Mieulet, Anne Diévart, Gaëtan Droc, Nadège Lanau, Emmanuel Guiderdoni
    Pages 205-221
  10. Transposon Display: A Versatile Method for Transposon Tagging

    • Michiel Vandenbussche, Jan Zethof, Tom Gerats
    Pages 239-250
  11. Massive Indexed Parallel Identification of Transposon Flanking Sequences

    • Michiel Vandenbussche, Jan Zethof, Tom Gerats
    Pages 251-264
  12. Use of Next Generation Sequencing (NGS) Technologies for the Genome-Wide Detection of Transposition

    • Moaine Elbaidouri, Cristian Chaparro, Olivier Panaud
    Pages 265-274

About this book

Transposable elements have played a major role in shaping plant genome structure and gene expression.  Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome.  In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.

Editors and Affiliations

  • Dept. Genetics, Development & Cell Biology, Iowa State University, Ames, USA

    Thomas Peterson

Bibliographic Information

  • Book Title: Plant Transposable Elements

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Peterson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-568-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-567-5Published: 06 August 2013

  • Softcover ISBN: 978-1-4939-6271-6Published: 23 August 2016

  • eBook ISBN: 978-1-62703-568-2Published: 13 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 324

  • Number of Illustrations: 38 b/w illustrations, 23 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access