Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB)
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Table of contents (25 protocols)
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Front Matter
Keywords
About this book
Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.
Reviews
From the reviews of the third edition:
“With this third edition, the authors and contributors continue to provide what has become a tour de force of bench top manuals for the cytochrome P450 field. … this book should also prove of interest to graduate students and postdoctoral researchers who are new to the field of P450 science, or who would like to expand their research directions. … This stands alone as the premier book for this field, which is sorely lacking in publications of this type.” (Jed N. Lampe, Doody’s Book Reviews, October, 2013)Editors and Affiliations
Bibliographic Information
Book Title: Cytochrome P450 Protocols
Editors: Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-321-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-320-6Published: 09 March 2013
Softcover ISBN: 978-1-4939-6269-3Published: 23 August 2016
eBook ISBN: 978-1-62703-321-3Published: 12 March 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XII, 319