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Cell Senescence

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Lorenzo Galluzzi

    1. Institut Gustave Roussy, Pavillon de Recherche 1, INSERM U848, Villejuif, France

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  2. Ilio Vitale

    1. Institut Gustave Roussy, Pavillon de Recherche 1, INSERM U848, Villejuif CEDEX, France

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  3. Oliver Kepp

    1. Institut Gustave Roussy, Pavillon de Recherche 1, INSERM U848, Villejuif CEDEX, France

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  4. Guido Kroemer

    1. Institut Gustave Roussy (IGR), Pavillon de Recherche 1, INSERM, U848, Villejuif, France

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  • Methods cover all biochemical and functional manifestations of senescence at the cellular level
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 965)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Cell Senescence as Both a Dynamic and a Static Phenotype

    • Andrew R. J. Young, Masako Narita, Masashi Narita
    Pages 1-13

  3. Senescence Regulation by mTOR

    • Vjekoslav Dulic
    Pages 15-35

  4. Senescence Regulation by the p53 Protein Family

    • Yingjuan Qian, Xinbin Chen
    Pages 37-61

  5. Markers of Cellular Senescence

    • Amancio Carnero
    Pages 63-81

  6. Biomarkers of Cell Senescence Assessed by Imaging Cytometry

    • Hong Zhao, Zbigniew Darzynkiewicz
    Pages 83-92

  7. Cytofluorometric Assessment of Cell Cycle Progression

    • Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier Metivier, Maria Castedo, Guido Kroemer
    Pages 93-120

  8. Quantification of Cell Cycle-Arresting Proteins

    • Oliver Kepp, Isabelle Martins, Laurie Menger, Mickaël Michaud, Sandy Adjemian, Abdul Qader Sukkurwala et al.
    Pages 121-142

  9. Colorimetric Detection of Senescence-Associated β Galactosidase

    • Koji Itahana, Yoko Itahana, Goberdhan P. Dimri
    Pages 143-156

  10. Chemiluminescent Detection of Senescence-Associated β Galactosidase

    • Vinicius Bassaneze, Ayumi Aurea Miyakawa, José Eduardo Krieger
    Pages 157-163

  11. Detection of the Senescence-Associated Secretory Phenotype (SASP)

    • Francis Rodier
    Pages 165-173

  12. Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Proteomics

    • Juan Carlos Acosta, Ambrosius P. Snijders, Jesús Gil
    Pages 175-184

  13. Detection of Senescence-Associated Heterochromatin Foci (SAHF)

    • Katherine M. Aird, Rugang Zhang
    Pages 185-196

  14. Monitoring DNA Damage During Cell Senescence

    • Glyn Nelson, Thomas von Zglinicki
    Pages 197-213

  15. Assessment and Quantification of Telomerase Enzyme Activity

    • Michelle F. Maritz, Laura A. Richards, Karen L. MacKenzie
    Pages 215-231

  16. Methods for the Assessment of Telomere Status

    • Asako J. Nakamura
    Pages 233-242

  17. Detection of Nuclear Envelope Alterations in Senescence

    • Clea Bárcena, Fernando G. Osorio, José Maria Pérez Freije
    Pages 243-251

  18. Measuring Reactive Oxygen Species in Senescent Cells

    • João F. Passos, Satomi Miwa, Thomas von Zglinicki
    Pages 253-263

  19. Quantification of Protein Carbonylation

    • Nancy B. Wehr, Rodney L. Levine
    Pages 265-281

  20. Assays for the Measurement of Lipid Peroxidation

    • Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, Neven Zarkovic
    Pages 283-296
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Keywords

About this book

 Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • Institut Gustave Roussy, Pavillon de Recherche 1, INSERM U848, Villejuif, France

    Lorenzo Galluzzi

  • Institut Gustave Roussy, Pavillon de Recherche 1, INSERM U848, Villejuif CEDEX, France

    Ilio Vitale, Oliver Kepp

  • Institut Gustave Roussy (IGR), Pavillon de Recherche 1, INSERM, U848, Villejuif, France

    Guido Kroemer

Bibliographic Information

  • Book Title: Cell Senescence

  • Book Subtitle: Methods and Protocols

  • Editors: Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-239-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Busincess Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-238-4Published: 08 January 2013

  • Softcover ISBN: 978-1-4939-5986-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-239-1Published: 09 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 538

  • Topics: Cell Biology, Cell Physiology

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