Editors:
- Detailed protocols for performing a particular procedure
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 849)
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Table of contents (35 protocols)
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Front Matter
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In Vitro Models and Assays
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Front Matter
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IN VITRO MODELS AND ASSAYS
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Cell Culture Models and Assays
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Front Matter
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About this book
Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word “amyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.
Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.
Editors and Affiliations
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School of Medicine, Dept. Psychiatry, New York University, New York, USA
Einar M. Sigurdsson
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Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain
Miguel Calero
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Inst. Química Física, Rocasolano, CSIC Madrid, Madrid, Spain
María Gasset
Bibliographic Information
Book Title: Amyloid Proteins
Book Subtitle: Methods and Protocols
Editors: Einar M. Sigurdsson, Miguel Calero, María Gasset
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-551-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-550-3Published: 24 February 2012
Softcover ISBN: 978-1-4939-5623-4Published: 23 August 2016
eBook ISBN: 978-1-61779-551-0Published: 23 April 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XV, 548
Number of Illustrations: 69 b/w illustrations, 46 illustrations in colour
Topics: Protein Science