The Blood-Brain and Other Neural Barriers

1. Front Matter

   Pages i-xiv

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2. Biology of the Barriers

   1. Front Matter

      Pages 1-1

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   2. Morphology and Properties of Brain Endothelial Cells

      • Sukriti Nag

      Pages 3-47

   3. Morphology and Properties of Pericytes

      • Paula Dore-Duffy, Kristen Cleary

      Pages 49-68

   4. Morphology and Properties of Astrocytes

      • Sukriti Nag

      Pages 69-100

   5. The Blood–Cerebrospinal Fluid Barrier: Structure and Functional Significance

      • Conrad E. Johanson, Edward G. Stopa, Paul N. McMillan

      Pages 101-131

   6. The Blood-Retinal Barrier: Structure and Functional Significance

      • E. Aaron Runkle, David A. Antonetti

      Pages 133-148

   7. The Blood-Nerve Barrier: Structure and Functional Significance

      • Ananda Weerasuriya, Andrew P. Mizisin

      Pages 149-173

3. Imaging the Barriers

   1. Front Matter

      Pages 175-175

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   2. Detection of Multiple Proteins in Intracerebral Vessels by Confocal Microscopy

      • Janet L. Manias, Anish Kapadia, Sukriti Nag

      Pages 177-192

   3. Multiparametric Magnetic Resonance Imaging and Repeated Measurements of Blood-Brain Barrier Permeability to Contrast Agents

      • Tavarekere N. Nagaraja, Robert A. Knight, James R. Ewing, Kishor Karki, Vijaya Nagesh, Joseph D. Fenstermacher

      Pages 193-212

   4. Detection of Brain Pathology by Magnetic Resonance Imaging of Iron Oxide Micro-particles

      • Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis, Robin P. Choudhury

      Pages 213-227

   5. Measuring the Integrity of the Human Blood–Brain Barrier Using Magnetic Resonance Imaging

      • Andrea Kassner, Rebecca Thornhill

      Pages 229-245

   6. Assessing Blood–Cerebrospinal Fluid Barrier Permeability in the Rat Embryo

      • Norman R. Saunders, C. Joakim Ek, Mark D. Habgood, Pia Johansson, Shane Liddelow, Katarzyna M. Dziegielewska

      Pages 247-265

   7. Detection of Blood–Nerve Barrier Permeability by Magnetic Resonance Imaging

      • Carsten Wessig

      Pages 267-271

4. Molecular Techniques to Study the Blood–Brain Barrier

   1. Front Matter

      Pages 273-273

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5. Molecular Techniques to Study the Blood-Brain Barrier

   1. Isolation of Human Brain Endothelial Cells and Characterization of Lipid Raft-Associated Proteins by Mass Spectroscopy

      • Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers, Alexandre Prat

      Pages 275-295

   2. Analysis of Mouse Brain Microvascular Endothelium Using Laser Capture Microdissection Coupled with Proteomics

      • Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan Lu, Shiaw-Lin Wu, William S. Hancock, Joel S. Pachter

      Pages 297-311

   3. Molecular and Functional Characterization of P-Glycoprotein In Vitro

      • Gary N. Y. Chan, Reina Bendayan

      Pages 313-336

   4. Methods to Study Glycoproteins at the Blood-Brain Barrier Using Mass Spectrometry

      • Arsalan S. Haqqani, Jennifer J. Hill, James Mullen, Danica B. Stanimirovic

      Pages 337-353

Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases. In The Blood-Brain and Other Neural Barriers: Reviews and Protocols, experts in the field present a series of cutting-edge protocols which can be used to study the barriers. Opening with detailed information on components of the neurovascular unit as well as the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, the book continues with meticulous techniques to image the barriers in humans and experimental animals, followed by cutting-edge molecular techniques to study the BBB and novel models to study the barriers, and it concludes with techniques for the delivery of therapeutic agents across the BBB. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, The Blood-Brain and Other Neural Barriers: Reviews and Protocols will aid the research efforts of not only graduate students but also more experienced investigators and support future studies of these vital systems.

• BBB breakdown
• Blood-brain barrier
• Blood-cerebrospinal barrier
• Blood-nerve barrier
• Blood-retinal barrier
• Brain endothelial cells
• Cerebral endothelium
• Pericytes, astrocytes, & neurons
• Therapeutic agents

• Banting Institute, Dept. Laboratory Med. & Pathobiology, University of Toronto, Toronto, Canada

  Sukriti Nag

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