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  • Book
  • © 2010

Carbon Nanotubes

Methods and Protocols

  • Presents functionalization protocols essential for implementing carbon nanotubes into molecular biology methods
  • Serves as a comprehensive guide to the use of carbon nanotubes in biochemical sensing, cytotoxicity testing, and gene delivery
  • Provides a comprehensive survey of how to employ functionalized carbon nanotubes in molecular biology studies
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 625)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Functionalization

    1. Front Matter

      Pages 1-1
    2. Non-covalent Attachment of Proteins to Single-Walled Carbon Nanotubes

      • Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan, David W. Schmidtke et al.
      Pages 3-8
    3. Covalent Conjugation of Multi-walled Carbon Nanotubes with Proteins

      • Changqing Yi, Suijian Qi, Dawei Zhang, Mengsu Yang
      Pages 9-17
    4. Covalently Linked Deoxyribonucleic Acid with Multi-walled Carbon Nanotubes: Synthesis and Characterization

      • Weiwei Chen, Changqing Yi, Tzang Chi-Hung, Shuit-Tong Lee, Mengsu Yang
      Pages 19-26
  3. Toxicity

    1. Front Matter

      Pages 39-39
    2. Effects of Carbon Nanotubes on the Proliferation and Differentiation of Primary Osteoblasts

      • Dawei Zhang, Changqing Yi, Suijian Qi, Xinsheng Yao, Mengsu Yang
      Pages 41-53
    3. Carbon Nanotube Uptake and Toxicity in the Brain

      • Leying Zhang, Darya Alizadeh, Behnam Badie
      Pages 55-65
    4. In Vitro and In Vivo Biocompatibility Testing of Functionalized Carbon Nanotubes

      • Gianni Ciofani, Vittoria Raffa, Orazio Vittorio, Alfred Cuschieri, Tommaso Pizzorusso, Mario Costa et al.
      Pages 67-83
    5. Real-Time Monitoring of Cellular Responses to Carbon Nanotubes

      • Qingxin Mu, Shumei Zhai, Bing Yan
      Pages 85-94
  4. Scaffolds

    1. Front Matter

      Pages 169-169
    2. Carbon Nanotube-Based Neurochips

      • Moshe David-Pur, Mark Shein, Yael Hanein
      Pages 171-177
    3. Effect of Carbon Nanotubes on HepG2 Adhesion and Spreading

      • Suijian Qi, Changqing Yi, Dawei Zhang, Mengsu Yang
      Pages 179-194

About this book

Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: Methods and Protocols provides reliable, consistent protocols on the application of CNTs in molecular biology-related fields. These are of vital importance, as the commercially available CNTs differ in purity, agglomeration state, as well as length and diameter distribution, all of which have a profound influence on the dispersability and surface properties of the tubes. The volume contains detailed sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, provided by expert researchers from various fields. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Carbon Nanotubes: Methods and Protocols serves to contribute to the achievement of common standards and helps researchers to avoid discrepancies in future biology-related CNT studies.

Reviews

From the reviews:

“A comprehensive introduction to the application of carbon nanotubes (CNTs) in the life-sciences. … it also provides detailed step-by-step procedures and complete lists of the reagents and materials required to carry out the applications introduced. … ‘Carbon Nanotubes: Methods and Protocols’ is an indispensable tool for researchers looking for a comprehensive summary of the state-of-the-art of applications of CNTs. … a valuable tool both for experts and for readers who intend to step into the fascinating world of CNT applications in biology and medicine.”­­­ (Frank Schleifenbaum, Analytical and Bioanalytical Chemistry, Vol. 399, October, 2010)

Editors and Affiliations

  • MPI für Festkörperforschung, Stuttgart, Germany

    Kannan Balasubramanian, Marko Burghard

Bibliographic Information

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access