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  • © 2008

Cell Fusion

Overviews and Methods

  • Comprehensive overviews of a variety of cell fusion systems
  • Most commonly-used techniques relevant to cell fusion in one volume
  • Classic and state-of-the-art methods in a readily producible format
  • Applications to cultured cells and different model organisms
  • Step-by-step instructions to ensure successful results
  • Tips on troubleshooting and avoiding known pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 475)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Cell Fusion

    1. Yeast Mating

      • Casey A. Ydenberg, Mark D. Rose
      Pages 3-20
    2. Cell Fusion in the Filamentous Fungus, Neurospora crassa

      • André Fleißner, Anna R. Simonin, N. Louise Glass
      Pages 21-38
    3. Cell Fusion in Caenorhabditis elegans

      • Scott Alper, Benjamin Podbilewicz
      Pages 53-74
    4. Myoblast Fusion in Drosophila

      • Susan M. Abmayr, Shufei Zhuang, Erika R. Geisbrecht
      Pages 75-97
    5. Mammalian Fertilization Is Dependent on Multiple Membrane Fusion Events*

      • Paul M. Wassarman, Eveline S. Litscher
      Pages 99-113
    6. Molecular Control of Mammalian Myoblast Fusion

      • Katie M. Jansen, Grace K. Pavlath
      Pages 115-133
    7. Placenta Trophoblast Fusion

      • Berthold Huppertz, Marcus Borges
      Pages 135-147
    8. Macrophage Fusion

      • Agnès Vignery
      Pages 149-161
  3. Cell Fusion Assays

    1. Ultrastructural Analysis of Cell Fusion in Yeast

      • Alison E. Gammie
      Pages 197-211
    2. Live Imaging of Drosophila Myoblast Fusion

      • Brian E. Richardson, Karen Beckett, Mary K. Baylies
      Pages 263-274
    3. Ultrastructural Analysis of Myoblast Fusion in Drosophila

      • Shiliang Zhang, Elizabeth H. Chen
      Pages 275-297
    4. A Genomic Approach to Myoblast Fusion in Drosophila

      • Beatriz Estrada, Alan M. Michelson
      Pages 299-314
    5. Mesenchymal Cell Fusion in the Sea Urchin Embryo

      • Paul G. Hodor, Charles A. Ettensohn
      Pages 315-334
    6. Sperm–Egg Fusion Assay in Mammals

      • Naokazu Inoue, Masaru Okabe
      Pages 335-345

About this book

Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events. In Cell Fusion: Overviews and Methods, a team of leading experts provide a collection of overviews that outline our current understanding of cell fusion and methods that present classic and state-of-the-art experimental approaches in a variety of systems.  Divided into two convenient parts, the volume begins with nine overviews which describe different cell fusion events in models from yeast to mammals, and it continues with thirteen chapters illustrating commonly used methods to assay cell fusion in particular systems. As a part of the highly successful Methods in Molecular Biologyâ„¢ series, these methods chapters compile step-by-step, readily reproducible protocols with lists of the necessary materials and reagents, along with tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and user-friendly, Cell Fusion: Overviews and Methods serves as a comprehensive resource for anyone, expert or novice, interested in the fascinating biological process of cell fusion.

Reviews

From the reviews:

"The book was planned to serve as ‘a comprehensive resource for anyone who is interested in this fascinating biological problem’. … a useful resource for the beginners and for those colleagues that are already involved with cell fusion topics. … the book is a very useful source on information, with a user-friendly format, for all scientists. … The technologies employed are so well described that for sure it will be possible even to beginning students to enter the field … ." (CarloAlberto Redi, European Journal of Histochemistry, Vol. 52 (4), December, 2008)

Editors and Affiliations

  • Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore

    Elizabeth H. Chen

Bibliographic Information

  • Book Title: Cell Fusion

  • Book Subtitle: Overviews and Methods

  • Editors: Elizabeth H. Chen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-250-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-911-6Published: 31 July 2008

  • Softcover ISBN: 978-1-61737-840-9Published: 19 November 2010

  • eBook ISBN: 978-1-59745-250-2Published: 03 November 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 421

  • Topics: Biochemistry, general, Cell Biology, Biological Techniques, Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access