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Plant Proteomics

Methods and Protocols

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  • © 2007
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Overview

Editors:
  1. Hervé Thiellement

    1. UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

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  2. Michel Zivy

    1. UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

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  3. Catherine Damerval

    1. UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

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  4. Valérie Méchin

    1. UMR de Chimie Biologique, Thiverval Grignon, France

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Part of the book series: Methods in Molecular Biology (MIMB, volume 355)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Total Protein Extraction with TCA-Acetone

    • Valérie Méchin, Catherine Damerval, Michel Zivy
    Pages 1-8
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  3. Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues

    • Mireille Faurobert, Esther Pelpoir, Jamila Chaïb
    Pages 9-14
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  4. Protein Extraction from Cereal Seeds

    • Gérard Branlard, Emmanuelle Bancel
    Pages 15-25
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  5. Protein Extraction from Xylem and Phloem Sap

    • Julia Kehr, Martijn Rep
    Pages 27-35
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  6. Protein Extraction from Woody Plants

    • Christophe Plomion, Céline Lalanne
    Pages 37-41
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  7. Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis

    • Klaas J. van Wijk, Jean-Benoit Peltier, Lisa Giacomelli
    Pages 43-48
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  8. Isolation and Subfractionation of Plant Mitochondria for Proteomic Analysis

    • Holger Eubel, Joshua L. Heazlewood, A. Harvey Millar
    Pages 49-62
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  9. Extraction of Nuclear Proteins from Root Meristematic Cells

    • Fernando González-Camacho, Francisco Javier Medina
    Pages 63-72
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  10. Extraction of Nuclear Proteins

    • Setsuko Komatsu
    Pages 73-77
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  11. Isolation of Cell Wall Proteins from Medicago sativa Stems

    • Bonnie S. Watson, Lloyd W. Sumner
    Pages 79-92
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  12. Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis

    • Véronique Santoni
    Pages 93-109
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  13. Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis

    • Thierry Rabilloud, Sylvie Luche, Véronique Santoni, Mireille Chevallet
    Pages 111-119
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  14. Two-Dimensional Electrophoresis for Plant Proteomics

    • Walter Weiss, Angelika Görg
    Pages 121-143
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  15. Visible and Fluorescent Staining of Two-Dimensional Gels

    • François Chevalier, Valérie Rofidal, Michel Rossignol
    Pages 145-156
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  16. Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum

    • Matthew Keeler, Jessica Letarte, Emily Hattrup, Fatimah Hickman, Paul A. Haynes
    Pages 157-174
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  17. Quantitative Analysis of 2D Gels

    • Michel Zivy
    Pages 175-194
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  18. Multivariate Data Analysis of Proteome Data

    • Kåre Engkilde, Susanne Jacobsen, Ib Søndergaard
    Pages 195-210
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  19. Edman Sequencing of Proteins from 2D Gels

    • Setsuko Komatsu
    Pages 211-217
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  20. Peptide Mass Fingerprinting

    • Nicolas Sommerer, Delphine Centeno, Michel Rossignol
    Pages 219-234
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Keywords

About this book

The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).

Editors and Affiliations

  • UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

    Hervé Thiellement, Michel Zivy, Catherine Damerval

  • UMR de Chimie Biologique, Thiverval Grignon, France

    Valérie Méchin

Bibliographic Information

  • Book Title: Plant Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Hervé Thiellement, Michel Zivy, Catherine Damerval, Valérie Méchin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597452270

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-635-1Published: 22 September 2006

  • Softcover ISBN: 978-1-61737-686-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-227-4Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 416

  • Number of Illustrations: 135 b/w illustrations

  • Topics: Plant Sciences

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