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Germ Cell Protocols

Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning

  • Book
  • © 2004

Overview

Editors:
  1. Heide Schatten

    1. Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 254)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos

    • Michele Boiani, James Kehler, Hans R. Schöler
    Pages 1-34

  3. Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans

    • Monika A. Jedrusik, Ekkehard Schulze
    Pages 35-48

  4. cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development

    • Myriam Hemberger
    Pages 49-65

  5. PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells

    • Thiagarajan Venkataraman, Elio Dancausse, Mary L. King
    Pages 67-78

  6. Differentiating Embryonic Stem Cells into Embryoid Bodies

    • Gisele Höpfl, Max Gassmann, Isabelle Desbaillets
    Pages 79-98

  7. The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation

    • Edward J. Gallagher, Jim McWhir
    Pages 99-111

  8. Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos

    • Jayne M. Squirrell, John G. White
    Pages 113-136

  9. Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes

    • Stephen A. Stricker
    Pages 137-148

  10. A Method for Producing Cloned Pigs by Using Somatic Cells as Donors

    • Liangxue Lai, Randall S. Prather
    Pages 149-163

  11. Pronuclear Microinjection of Mouse Zygotes

    • Thomas Rülicke
    Pages 165-194

  12. Parthenogenesis and Nuclear Transfer in Rabbit Oocytes

    • Yukio Tsunoda, Yoko Kato
    Pages 195-211

  13. Nuclear Transfer in Cattle

    • Gottfried Brem, Manzoor A. Nowshari
    Pages 213-225

  14. In Vitro Fertilization and Embryo Transfer in Felids

    • Charles Earle Pope
    Pages 227-244

  15. Production of Chimeric Chickens

    • Mitsuru Naito, Takashi Kuwana
    Pages 245-253

  16. Production of Haploid and Diploid Androgenetic Zebrafish

    • Bruce P. Brandhorst, Graham E. Corley-Smith
    Pages 255-270

  17. Construction and Detection of Fluorescent, Germline Transgenic Zebrafish

    • Elwood Linney, Ava J. Udvadia
    Pages 271-288

  18. Production of Zebrafish Germline Chimeras from Cultured Cells

    • Lianchun Fan, Annette Alestrom, Peter Alestrom, Paul Collodi
    Pages 289-299

  19. Studying Nuclear Reprogramming with Cell Hybrids

    • Steve Pells, Jim McWhir
    Pages 301-311

  20. Cytoplasmic Transfer in Mammalian Eggs and Embryos

    • Henry E. Malter
    Pages 313-324
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About this book

The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits st- ies of fertility and infertility in mammals including humans. External in vitro fertilization, now a common technique in assisted fertilization has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex that carry to- potency, an ability to develop into an entire new organism. The two volumes on germ cells combine techniques in a variety of different systems and have selected those systems that have provided landmarks in advancing our kno- edge on germ cells.

Editors and Affiliations

  • Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia

    Heide Schatten

Bibliographic Information

  • Book Title: Germ Cell Protocols

  • Book Subtitle: Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning

  • Editors: Heide Schatten

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597416

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-257-5Published: 24 March 2004

  • Softcover ISBN: 978-1-61737-457-9Published: 06 January 2011

  • eBook ISBN: 978-1-59259-741-3Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 418

  • Topics: Cell Biology

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