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Regulatory Protein Modification

Techniques and Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Hugh C. Hemmings

    1. Cornell University Medical College, New York

    View editor publications

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Part of the book series: Neuromethods (NM, volume 30)

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Table of contents (11 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Analysis of Protein Phosphorylation in Intact Cells and Extracts

    • S. Ivar Walaas, Anne Carine Østvold
    Pages 1-43

  3. Analysis of Protein Dephosphorylation in Intact Cells and Extracts

    • Shelley Halpain
    Pages 45-65

  4. Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes)

    • Talvinder S. Sihra
    Pages 67-119

  5. Protein Kinase and Phosphatase Inhibitors

    • Hugh C. Hemmings Jr.
    Pages 121-218

  6. Phosphorylation State-Specific Antibodies

    • Andrew J. Czernik, Jeffrey Mathers, Sheenah M. Mische
    Pages 219-250

  7. Protein Tyrosine Phosphorylation

    • Pascal Derkinderen, Jean-Antoine Girault
    Pages 251-274

  8. Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis

    • James A. Bibb, Edgar da Cruz e Silva F.
    Pages 275-307

  9. Protein methylation in the nervous system

    • Darin J. Weber, Philip N. McFadden
    Pages 309-333

  10. Long-Chain Fatty Acylation of Proteins

    • Sean I. Patterson, J. H. Pate Skene
    Pages 335-364

  11. Protein ADP-Ribosylation

    • Keith D. Philibert, Henk Zwiers
    Pages 365-394

  12. Glycosylation and Glycosylphosphatidylinositol Membrane Anchors

    • Anthony J. Turner, Edward T. Parkin, Nigel M. Hooper
    Pages 395-426

  13. Back Matter

    Pages 429-445
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Keywords

About this book

Hugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also
offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today.

Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.

Editors and Affiliations

  • Cornell University Medical College, New York

    Hugh C. Hemmings

Bibliographic Information

  • Book Title: Regulatory Protein Modification

  • Book Subtitle: Techniques and Protocols

  • Editors: Hugh C. Hemmings

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1385/0896034151

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1997

  • Hardcover ISBN: 978-0-89603-415-0Published: 01 December 1996

  • Softcover ISBN: 978-1-61737-030-4Published: 09 November 2010

  • eBook ISBN: 978-1-59259-635-5Published: 06 February 2008

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 446

  • Topics: Neurosciences

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