Embryonic Stem Cells

1. Front Matter

   Pages i-xvi

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2. Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells

   • Marsha L. Roach, John D. McNeish

   Pages 1-16

3. The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos

   • Gabriele Proetzel, Michael V. Wiles

   Pages 17-26

4. Analysis of the Cell Cycle in Mouse Embryonic Stem Cells

   • Pierre Savatier, Hélène Lapillonne, Ludmila Jirmanova, Luigi Vitelli, Jacques Samarut

   Pages 27-33

5. Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation

   • André-Patrick Arrigo, Patrick Mehlen

   Pages 35-44

6. Effects of Altered Gene Expression on ES Cell Differentiation

   • Yong Fan, J. Richard Chaillet

   Pages 45-53

7. Hypoxic Gene Regulation in Differentiating ES Cells

   • David M. Adelman, M. Celeste Simon

   Pages 55-62

8. Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells

   • Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, Tetsuro Takamatsu

   Pages 63-69

9. Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development

   • Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, Shin-Ichi Nishikawa

   Pages 71-81

10. Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System

    • Takumi Era, Stephane Wong, Owen N. Witte

    Pages 83-95

11. Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development

    • Toshiyuki Yamane, Takahiro Kunisada, Shin-Ichi Hayashi

    Pages 97-106

12. Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells

    • Christian Dani

    Pages 107-116

13. Embryonic Stem Cell Differentiation and the Vascular Lineage

    • Victoria L. Bautch

    Pages 117-125

14. Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation

    • Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, Kenneth R. Boheler

    Pages 127-156

15. Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications

    • Kishore B. S. Pasumarthi, Loren J. Field

    Pages 157-168

16. Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System

    • Jüyrgen Hescheler, Maria Wartenberg, Bernd K. Fleischmann, Kathrin Banach, Helmut Acker, Heinrich Sauer

    Pages 169-187

17. Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells

    • Tahmina Mujtaba, Mahendra S. Rao

    Pages 189-204

18. Lineage Selection for Generation and Amplification of Neural Precursor Cells

    • Meng Li

    Pages 205-215

19. Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson's Disease

    • Hiroshi Kawasaki, Kenji Mizuseki, Yoshiki Sasai

    Pages 217-227

20. Epidermal Lineage

    • Tammy-Claire Troy, Kursad Turksen

    Pages 229-253

It is fair to say that embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day. It also seems certain that as this millennium unfolds we will see an increase in scientific and ethical debate about their potential utility in society. On the scientific front, it is clear that work on ES cells has already generated new possibilities and stimulated development of new strategies for increasing our und- standing of cell lineages and differentiation. It is not naïve to think that, within a decade or so, our overall understanding of stem cell biology will be as revolutionized as it was when the pioneering hemopoietic stem cell studies of Till and McCulloch in Toronto captured our imaginations in 1961. With it will come better methods for ES and lineage-specific stem cell identification, maintenance, and controlled fate selection. Clearly, ES cell models are already providing opportunities for the est- lishment of limitless sources of specific cell populations. In recognition of the gr- ing excitement and potential of ES cells as models for both the advancement of basic science and future clinical applications, I felt it timely to edit this collection of pro- cols (Embryonic Stem Cells) in which forefront investigators would provide detailed methods for use of ES cells to study various lineages and tissue types.

"The book could be used effectively by experienced or novice researchers in the field since the protocols are written clearly and concisely. There are 35 brief chapters in this book by over 80 authors who are experienced and knowledgeable researchers in the field...Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing ES cells from a variety of experimental animals. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture ES cells and/or apply the specialized techniques for studying them. There is an adequate index. This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on ES cells." -Doody's Health Sciences Book Review Journal

"...this book provides a good overview of work being done in the development of protocols for culture, controlled differentiation and manipulation of ES cells" - Newsletter of the British Society of Developmental Biology

"Embryonic Stem Cells Methods and Protocols is one of the most useful books I have ever read in this field, and the awesome note at he end of each chapter makes it a great work. It represents good value for money and should be useful as a reference book." - Cell Biology International

"...a valuable collection of up-to-date protocols written by leading experts in the field. It is well written and clearly structured. The volume appears highly valuable for any scientist who wants to make a start in this exciting field but also for experienced ES cell researchers who want to widen their repertoire. In addition, the protocols involving hematopoietic, adipocyte and vascular differentiation could easily be adopted by diabetologists to promote our understanding of cell systems which are of special interest in diabetes research." - Diabetologia

"This book provides a very informative resource forany developmental or cell biologist with an interest in developments and prospects of ES cell research." -Molecular Biotechnology

• Ottawa Health Research Institute, Ottawa, Canada

  Kursad Turksen

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