Capillary Electrophoresis of Nucleic Acids

1. Front Matter

   Pages i-xx

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2. Practical Applications for Rapid DNA Fragment Sizing and Analysis

   1. Front Matter

      Pages 3-45

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   2. Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis

      • H. Michael Wenz, David Dailey, Martin D. Johnson

      Pages 3-17

   3. Ultra-Fast DNA Separations Using Capillary Electrophoresis

      • Karel Klepárník, Odilo M. Müeller, František Foret

      Pages 19-39

   4. Fast DNA Fragment Sizing in a Short Capillary Column

      • Haleem J. Issaq, King C. Chan

      Pages 41-45

3. Practical Applications for Mutation Detection

   1. Front Matter

      Pages 47-147

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   2. Detection of DNA Polymorphisms Using PCR-RFLP and Capillary Electrophoresis

      • John M. Butler, Dennis J. Reeder

      Pages 49-56

   3. High Resolution Analysis of Point Mutations by Constant Denaturant Capillary Electrophoresis (CDCE)

      • Konstantin Khrapko, Hilary A. Coller, Xiao-Cheng Li-Sucholeiki, Paulo C. André, William G. Thilly

      Pages 57-72

   4. Point Mutation Detection by Temperature-Programmed Capillary Electrophoresis

      • Cecilia Gelfi, Laura Cremoresi, Maurizio Ferrari, Pier Giorgio Righetti

      Pages 73-88

   5. Single-Nucleotide Primer Extension Assay by Capillary Electrophoresis Laser-Induced Fluorescence

      • Christine A. Piggee, Barry L. Karger

      Pages 89-94

   6. Amplification Refractory Mutation System Analysis of Point Mutations by Capillary Electrophoresis

      • Paola Carrera, Pier Giorgio Righetti, Cecilia Gelfi, Maurizio Ferrari

      Pages 95-108

   7. SSCP Analysis of Point Mutations by Multicolor Capillary Electrophoresis

      • Kenshi Hayashi, H. Michael Wenz, Masakazu Inazuka, Tomoko Tahira, Tomonari Sasaki, Donald H. Atha

      Pages 109-126

   8. SSCP Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detector

      • Jicun Ren

      Pages 127-134

   9. Magnetic Bead-Isolated Single-Strand DNA for SSCP Analysis

      • Takao Kasuga, Jing Cheng, Keith R. Mitchelson

      Pages 135-147

4. Practical Applications for Genetic Analysis

   1. Front Matter

      Pages 149-239

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   2. Analysis of Short Tandem Repeat Markers by Capillary Array Electrophoresis

      • Elaine S. Mansfield, Robert B. Wilson, Paolo Fortina

      Pages 151-161

   3. Genotyping by Microdevice Electrophoresis

      • Dieter Schmalzing, Lance Koutny, Aram Adourian, Dan Chisholm, Paul Matsudaira, Daniel Ehrlich

      Pages 163-173

   4. Applications of Constant Denaturant Capillary Electrophoresis and Complementary Procedures

      • Andrea S. Kim, Xiao-Cheng Li-Sucholeiki, William G. Thilly

      Pages 175-189

   5. Toward Effective PCR-Based Amplification of DNA on Microfabricated Chips

      • Jerome P. Ferrance, Braden Giordano, James P. Landers

      Pages 191-204

   6. Low-Stringency Single Specific Primer-Amplified DNA Analysis by Capillary Electrophoresis

      • Michael A. Marino

      Pages 205-210

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single–strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

• DNA
• Nucleotide
• PCR
• RNA
• fluorescence
• gene expression
• temperature
• tissue

"These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes)....Chapters on the use of microchip and mass spectrometric detection are also included....Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE. ...Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE." - Clinical Chemistry

• Australian Genome Research Facility, University of Queensland, Brisbane

  Keith R. Mitchelson

• Walter & Eliza Hall Institute, Parkville, Australia

  Keith R. Mitchelson

• Biochip Research and Development Center State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

  Jing Cheng

• Aviva Biosciences Corporation, San Diego

  Jing Cheng

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