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  • Book
  • © 2001

Angiotensin Protocols

Editors:

  1. Donna H. Wang

    1. Department of Medicine, Michigan State University, East Lansing

    View editor publications

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 51)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Introduction to The Renin-Angiotensin System

    1. Front Matter

      Pages 1-1
      PDF

    3. Three Angiotensin Paradigms in One Patient

      • Cheryl L. Laffer, Fernando Elijovich
      Pages 23-37

  3. Transgenic and Gene Targeting Methods

    1. Front Matter

      Pages 39-39
      PDF

    2. Production and Use of Chimeric Mice

      • Junji Takaya, Taiji Matsusaka, Iekuni Ichikawa
      Pages 41-52

    4. Systemic Delivery of aTransgene in Intact Animals by a Retroviral Vector

      • Michael J. Katovich, Hong-Wei Wang, Craig H. Gelband, Mohan K. Raizada
      Pages 67-81

    5. Antisense Inhibition of the Renin-Angiotensin System

      • Dagmara Mohuczy, M. Ian Phillips
      Pages 83-104

  4. Analysis of Transcriptional and Translational Mechanisms

    1. Front Matter

      Pages 105-105
      PDF

    2. A nalysis of Transcriptional Control Mechanisms I

      • Christopher T. Sherman, Allan R. Brasier
      Pages 107-125

    3. Analysis of Transcriptional Control Mechanisms II Techniques for Characterization of trans-Acting Factors

      • Allan R. Brasier, Christopher T. Sherman, Mohammad Jamaluddin
      Pages 127-150

    5. In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts

      • Hong Ji, Kamakshi Krishnamurthi, Zheng Wu, Kathryn Sandberg
      Pages 171-192

  5. Assays For Determination of Mrna Levels

    1. Front Matter

      Pages 193-193
      PDF

    3. Analysis of RNA by Northern-Blot Hybridization

      • Patricia E. Gallagher, Debra I. Diz
      Pages 205-213

    5. In Situ Hybridization

      • Susan Riggs Runge, Zhaoyong Hu, Marschall S. Runge
      Pages 223-233

  6. Assays For Determination of Protein Contents and Enzyme Activity

    1. Front Matter

      Pages 235-235
      PDF
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About this book

A qualitative leap in the understanding of cardiovascular and n- ral regulation by the renin–angiotensin system, and of the role of this s- tem in tissue damage, has occurred as a result of the many recent advances in molecular genetic techniques. The cloning of the genes for the components of the renin–angiotensin system, the design of specific angiotensin receptor ligands, and the use of embryonic gene targeting te- niques for the creation of mutant strains have established that the renin– angiotensin system is important in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling Further investigation of the mechanisms by which this system p- ticipates in cardiovascular regulation may shed some light on the pat- genesis of several cardiovascular diseases, e. g. , hypertension, congestive heart failure, and chronic renal failure. Despite the promise of this system as a target for therapeutic interventions for these diseases, there are great challenges in the integration of the attempts to close the gap between the traditional literature of medicine and the explosion of information from the new technologies. This book’s title, Angiotensin Protocols, reflects the authors’ strong efforts to translate expert knowledge into easy-to-follow practice. The book opens with introductory chapters, and each specialty section provides detailed methods covering a wide variety of techniques, ranging from genetic manipulation of targeted genes to functional studies of the renin– angiotensin system.
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Reviews

"This is an advanced treatise on the renin-angiotensin system (RAS) and its regulation of the cardiovascular, renal, and neural relationships in health and disease. The introduction is ample to move the biological scientist reader up to adequacy to comprehend a complex patient example. ...The purpose shows a goal along with suitable objectives to move traditional medicine into utilization of the amazing explosion of modern biological techniques in RAS applications. It does so with a compilation of experts that foster a clearer picture of the modem RAS field. These are indeed worthy objectives, that surpass what individual papers and review articles can accomplish. Indeed it is needed and it serves as a tremendous thinking stimulator....The introduction by John E. Hall sets the tone and makes this book happen. The genetic manipulation of the RAS by Sigmund and Stec keeps the happening alive. the quantification of angiotensin II receptors by Wu, Yao and Wang is an outstanding peace of work. The cyclic AMP and GMP by Ji was helpful as was the NO from assay work of Thorup and Moore. The closing article on cerebral circulation by Lombard carries the exciting nature of the book to the interested student."-Doody's Health Sciences Book Review Journal

"This book with great attention to the recent developments in molecular genetic, biochemical and fysiological techniques can be recommended to all researchers involved in the fundemental research of the RAS at the cellular level." - Acta Cardiologica
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Editors and Affiliations

  • Department of Medicine, Michigan State University, East Lansing

    Donna H. Wang

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Bibliographic Information

  • Book Title: Angiotensin Protocols

  • Editors: Donna H. Wang

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/159259087X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-763-2Published: 29 October 2000

  • Softcover ISBN: 978-1-61737-176-9Published: 12 December 2011

  • eBook ISBN: 978-1-59259-087-2Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 530

  • Topics: Human Physiology

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

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