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  • © 2000

Aqueous Two-Phase Systems

Methods and Protocols

Editors:

  1. Rajni Hatti-Kaul

    1. Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

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Part of the book series: Methods in Biotechnology (MIBT, volume 11)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xiii
    PDF

  2. Preparation and Characterization of Aqueous Two-Phase Systems

    1. Aqueous Two-Phase Systems

      • Rajni Hatti-Kaul
      Pages 1-10

    2. The Phase Diagram

      • Anita Kaul
      Pages 11-21

    3. Preparation of Aqueous Two-Phase Systems

      • Daniel Forciniti
      Pages 23-33

    4. Measurement of Some Physical Properties of Aqueous Two-Phase Systems

      • Donald E. Brooks, Raymond Norris Jones
      Pages 35-45

  3. Partitioning Techniques

    1. Front Matter

      Pages 11-45
      PDF

    2. Single-Step Partitioning in Aqueous Two-Phase Systems

      • Telma Teixeira Franco, Rajni Hatti-Kaul
      Pages 47-54

    3. Partition by Countercurrent Distribution (CCD)

      • Hans-Erik Åkerlund
      Pages 55-64

    4. Liquid-Liquid Partition Chromatography (LLPC)

      • Ulla-Britt Hansson, Christer Wingren
      Pages 65-75

  4. Partitioning of Soluble Molecules and Particulates

    1. Front Matter

      Pages 46-75
      PDF

    2. Metal Ion Separations in Aqueous Biphasic Systems and with ABEC™ Resins

      • Jonathan G. Huddleston, Scott T. Griffin, Jianhua Zhang, Heather D. Willauer, Robin D. Rogers
      Pages 77-94

    5. Eukaryotic Cell Partition

      • James M. Van Alstine
      Pages 119-142

    6. Concentration and Purification of Viruses

      • Lena Hammar
      Pages 143-158

    7. Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles

      • Christer Larsson, Susanne Widell
      Pages 159-166

    8. Isolation of Inside-Out Thylakoid Vesicles

      • Hans-Erik Åkerlund
      Pages 167-175

    11. Purification of Plasma Membranes by Affinity Partitioning

      • Bengt Jergil, Lars Ekblad
      Pages 193-200
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About this book

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
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Editors and Affiliations

  • Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

    Rajni Hatti-Kaul

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Bibliographic Information

  • Book Title: Aqueous Two-Phase Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Rajni Hatti-Kaul

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1385/1592590284

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-541-6Published: 21 February 2000

  • Softcover ISBN: 978-1-61737-067-0Published: 19 November 2010

  • eBook ISBN: 978-1-59259-028-5Published: 05 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XIV, 454

  • Topics: Biotechnology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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