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TRP Channels

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Antonio Ferrer-Montiel

    1. Instituto de Biología, Molecular y Celular, Universidad Miguel Hernández, Elche, Spain

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  2. Tim Hucho

    1. Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1987)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Molecular Evolution Bioinformatics Toward Structural Biology of TRPV1-4 Channels

    • Pau Doñate-Macián, Alba Crespi-Boixader, Alex Perálvarez-MarĂ­n
    Pages 1-21

  3. Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6

    • Appu K. Singh, Luke L. McGoldrick, Alexander I. Sobolevsky
    Pages 23-37

  4. Cryo Electron Microscopy of TRP Channels

    • Amrita Samanta, Taylor E. T. Hughes, Vera Y. Moiseenkova-Bell
    Pages 39-50

  5. Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation

    • VerĂłnica I. Dumit, Michael Köttgen, Alexis Hofherr
    Pages 51-64

  6. Combining Structural Data with Computational Methodologies to Investigate Structure–Function Relationships in TRP Channels

    • Victoria Oakes, Carmen Domene
    Pages 65-82

  7. Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique

    • Elvira de la Peña, Ana Gomis
    Pages 83-97

  8. Fluorescence-Based Functional Assays for Ca2+-Permeable ThermoTRP Channels

    • Celia Cordero-Sánchez, Irene Mudarra-Fraguas, Asia Fernández-Carvajal
    Pages 99-110

  9. High-Content Imaging of Immunofluorescently Labeled TRPV1-Positive Sensory Neurons

    • Jörg Isensee, Tim Hucho
    Pages 111-124

  10. Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels

    • Beiying Liu, Feng Qin
    Pages 125-141

  11. TRP Channel Reconstitution in Lipid Bilayers

    • Eleonora Zakharian
    Pages 143-166

  12. Methods for Investigating TRP Channel Gating

    • Osvaldo Alvarez, Karen Castillo, Emerson Carmona, Carlos Gonzalez, Ramon Latorre
    Pages 167-185

  13. In Silico Approaches for TRP Channel Modulation

    • Magdalena Nikolaeva Koleva, Gregorio Fernandez-Ballester
    Pages 187-206

  14. Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction

    • Tracey Pirali, Ubaldina Galli, Marta Serafini, Alessia Griglio, Armando A. Genazzani, Gian Cesare Tron
    Pages 207-221

  15. In Vivo Methods to Study ThermoTRP Channels in Rodents

    • Sara González-RodrĂ­guez
    Pages 223-231

  16. Back Matter

    Pages 233-238
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Keywords

About this book

This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab. 

Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels.

Editors and Affiliations

  • Instituto de BiologĂ­a, Molecular y Celular, Universidad Miguel Hernández, Elche, Spain

    Antonio Ferrer-Montiel

  • Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany

    Tim Hucho

Bibliographic Information

  • Book Title: TRP Channels

  • Book Subtitle: Methods and Protocols

  • Editors: Antonio Ferrer-Montiel, Tim Hucho

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9446-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9445-8Published: 27 April 2019

  • eBook ISBN: 978-1-4939-9446-5Published: 26 April 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 238

  • Number of Illustrations: 11 b/w illustrations, 58 illustrations in colour

  • Topics: Biochemistry, general, Pharmaceutical Sciences/Technology

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