Skip to main content
SpringerLink
Log in
Book cover

Post-Translational Modification of Proteins

Tools for Functional Proteomics

  • Book
  • © 2019

Overview

Editors:
  1. Christoph Kannicht

    1. Recombinant Research and Development, Octapharma Biopharmaceuticals GmbH, Heidelberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1934)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Web-Based Computational Tools for the Prediction and Analysis of Posttranslational Modifications of Proteins

    • Vladimir A. Ivanisenko, Timofey V. Ivanisenko, Olga V. Saik, Pavel S. Demenkov, Dmitry A. Afonnikov, Nikolay A. Kolchanov
    Pages 1-20

  3. Detection of Posttranslational Modifications by Fluorescent Staining of Two-Dimensional Gels

    • Archana M. Jastorff, Chris W. Turck
    Pages 21-32

  4. Analysis of Ser/Thr-Linked Sugar Chains

    • Kalyan R. Anumula
    Pages 33-42

  5. Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling

    • Detlef Grunow, Véronique Blanchard
    Pages 43-49

  6. Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures

    • Christoph Kannicht, Detlef Grunow, Lothar Lucka
    Pages 51-64

  7. Chromatographic Profiling of N-Glycans

    • Martin Gohlke, Véronique Blanchard
    Pages 65-81

  8. Activity Determination of Glycosyltransferases

    • Kalyan R. Anumula
    Pages 83-92

  9. Characterization of Site-Specific N-Glycosylation

    • Helga Hevér, Zsuzsanna Darula, Katalin F. Medzihradszky
    Pages 93-125

  10. Glycosylation of Type I Collagen

    • Mitsuo Yamauchi, Marnisa Sricholpech, Masahiko Terajima, Kenneth B. Tomer, Irina Perdivara
    Pages 127-144

  11. Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa

    • Nahid Azzouz, Peter Gerold, Ralph T. Schwarz
    Pages 145-162

  12. Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods

    • Christof Lenz
    Pages 163-178

  13. Mass Spectrometry-Based Method for Detection and Identification of Free Thiol Groups in Proteins

    • Barbara A. Solecka-Witulska, Christoph Weise, Christoph Kannicht
    Pages 179-189

  14. Mass Spectrometric Determination of Protein Ubiquitination

    • Carol E. Parker, Maria R. E. Warren Hines, Viorel Mocanu, Susanna F. Greer, Christoph H. Borchers
    Pages 191-221

  15. Analysis of Sumoylation

    • Jan Breucker, Andrea Pichler
    Pages 223-233

  16. An Approach for the Identification of Proteins Modified with ISG15

    • Tomoharu Takeuchi, Shun Koinuma, Hideyoshi Yokosawa, Yoichiro Arata
    Pages 235-246

  17. α-Amidated Peptides: Approaches for Analysis

    • Gregory P. Mueller, Rachel C. Lazarus, William J. Driscoll
    Pages 247-264

  18. S-Acylation of Proteins

    • Larisa Kordyukova, Ludwig Krabben, Marina Serebryakova, Michael Veit
    Pages 265-291

  19. Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins

    • Randy J. Arnold, Suraj Saraswat, James P. Reilly
    Pages 293-307

  20. Lysine Hydroxylation and Cross-Linking of Collagen

    • Mitsuo Yamauchi, Masahiko Terajima, Masashi Shiiba
    Pages 309-324
Back to top

Keywords

About this book

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins.  In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics,Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Editors and Affiliations

  • Recombinant Research and Development, Octapharma Biopharmaceuticals GmbH, Heidelberg, Germany

    Christoph Kannicht

Bibliographic Information

  • Book Title: Post-Translational Modification of Proteins

  • Book Subtitle: Tools for Functional Proteomics

  • Editors: Christoph Kannicht

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9055-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9053-5Published: 02 July 2019

  • eBook ISBN: 978-1-4939-9055-9Published: 29 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 327

  • Number of Illustrations: 81 b/w illustrations, 31 illustrations in colour

  • Topics: Posttranslational Modification, Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation