Skip to main content
SpringerLink
Log in
Book cover

Synaptosomes

  • Book
  • © 2018

Overview

Editors:
  1. Kathryn M. Murphy

    1. Department of Psychology, Neuroscience & Behavior, McMaster University, Hamilton, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 141)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Introduction to Synaptosomes

      • Kathryn M. Murphy
      Pages 3-6

  3. History of Synaptosomes

    1. Front Matter

      Pages 7-7
      Download chapter PDF

    2. Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications

      • Herbert Zimmermann
      Pages 9-26

    3. Synaptosome Preparations: Which Procedure Should I Use?

      • Peter R. Dunkley, Phillip J. Robinson
      Pages 27-53

  4. Making Synaptosomes

    1. Front Matter

      Pages 55-55
      Download chapter PDF

    2. Protocol for a High-Throughput Semiautomated Preparation for Filtered Synaptoneurosomes

      • Justin L. Balsor, Kathryn M. Murphy
      Pages 57-73

    3. Subcellular Fractionation of Brain Tissue from Small Tissue Explants

      • Véronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, Maria Florencia Angelo
      Pages 75-84

    4. Preparation of P2 or Percoll-Purified Synaptosomes from Mammalian Brain Tissue

      • Jing Xue, Annie Quan, Phillip J. Robinson
      Pages 85-105

  5. Studying Synaptic Energy and Metabolism Using Synaptosomes

    1. Front Matter

      Pages 107-107
      Download chapter PDF

    2. Synaptosomal Bioenergetics and Glutamate Release

      • David G. Nicholls
      Pages 109-129

    3. Synaptosome Bioenergetics and Calcium Handling: Aging Response

      • Silvia Lores-Arnaiz, Georgina Rodríguez de Lores Arnaiz, Analía G. Karadayian, Juanita Bustamante
      Pages 131-151

    4. Isolation of Highly Purified and Functionally Active Synaptic Mitochondria

      • Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin et al.
      Pages 153-163

    5. Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes

      • Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson et al.
      Pages 165-191

  6. Studying Synaptic Proteomics, Function, and Plasticity Using Synaptosomes

    1. Front Matter

      Pages 193-193
      Download chapter PDF

    2. Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein’s Effect on Neurotransmitter Release

      • Marianne Cilluffo, Theodore A. Sarafian, Joseph B. Watson
      Pages 195-208

    3. Porosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion, and Neurotransmitter Release

      • Bhanu P. Jena
      Pages 209-225

    4. SMPL Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses

      • Riana Lo Bu, Jeffrey Clark, Thomas DiChiara, Stephen G. Sligar, William L. Klein
      Pages 227-250

  7. Studying Development and Disease Using Synaptosomes

    1. Front Matter

      Pages 251-251
      Download chapter PDF
Back to top

Keywords

About this book

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

 
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.


Editors and Affiliations

  • Department of Psychology, Neuroscience & Behavior, McMaster University, Hamilton, Canada

    Kathryn M. Murphy

Bibliographic Information

  • Book Title: Synaptosomes

  • Editors: Kathryn M. Murphy

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-8739-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8738-2Published: 22 September 2018

  • Softcover ISBN: 978-1-4939-9379-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8739-9Published: 21 September 2018

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XV, 312

  • Number of Illustrations: 23 b/w illustrations, 32 illustrations in colour

  • Topics: Neurosciences

Publish with us

Policies and ethics

Back to top

Search

Navigation