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  • © 2018

Type 2 Immunity

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1799)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
  2. A Fungal Protease Model to Interrogate Allergic Lung Immunity

    • J. Morgan Knight, Evan Li, Hui-Ying Tung, Cameron Landers, Jake Wheeler, Farrah Kheradmand et al.
    Pages 1-9
  3. Induction and Characterization of the Allergic Eye Disease Mouse Model

    • Nancy J. Reyes, Rose Mathew, Daniel R. Saban
    Pages 49-57
  4. Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung

    • Hideki Nakano, Keiko Nakano, Donald N. Cook
    Pages 59-69
  5. In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation

    • Hae Woong Choi, Soman N. Abraham
    Pages 71-80
  6. The Use of Human and Mouse Mast Cell and Basophil Cultures to Assess Type 2 Inflammation

    • Heather L. Caslin, Marcela T. Taruselli, Anuya Paranjape, Kasalina Kiwanuka, Tamara Haque, Alena P. Chumanevich et al.
    Pages 81-92
  7. Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation

    • Jesse Charles Nussbaum, Jorge Felipe Ortiz-Carpena
    Pages 93-107
  8. Characterization of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity

    • Wanjiang Zeng, Rebecca L. O’Brien, Willi K. Born, Yafei Huang
    Pages 135-151
  9. The Identification of Allergen-Derived T Cell Epitopes

    • Véronique Schulten, Alessandro Sette
    Pages 153-163
  10. The Generation and Use of Allergen-Specific TCR Transgenic Animals

    • Manon Vanheerswynghels, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad et al.
    Pages 183-210
  11. Using Cytokine Reporter Mice to Visualize Type-2 Immunity In Vivo

    • Mark Dell’Aringa, R. Lee Reinhardt
    Pages 211-223
  12. Live Imaging of IL-4-Expressing T Follicular Helper Cells in Explanted Lymph Nodes

    • Mark Dell’Aringa, R. Lee Reinhardt, Rachel S. Friedman, Jordan Jacobelli
    Pages 225-235
  13. Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking

    • Miranda R. Lyons-Cohen, Hideki Nakano, Seddon Y. Thomas, Donald N. Cook
    Pages 237-246
  14. Study of IgE-Producing B Cells Using the Verigem Fluorescent Reporter Mouse

    • Zhiyong Yang, James B. Jung, Christopher D. C. Allen
    Pages 247-264

About this book

This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.

Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside. 

Editors and Affiliations

  • Department of Biomedical Research, National Jewish Health, Denver, USA

    R. Lee Reinhardt

Bibliographic Information

  • Book Title: Type 2 Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: R. Lee Reinhardt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7896-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7895-3Published: 29 June 2018

  • Softcover ISBN: 978-1-4939-9318-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7896-0Published: 28 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 434

  • Number of Illustrations: 27 b/w illustrations, 53 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access