Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1712)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
About this book
This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications, method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Next Generation Sequencing: Methodsand Protocols aims to be useful and informative for further study into this vital field.
Editors and Affiliations
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Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, USA
Steven R. Head, Phillip Ordoukhanian
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Department of Molecular Medicine, The Scripps Research Institute Center for Organ and Cell Transplantation, La Jolla, USA
Daniel R. Salomon
Bibliographic Information
Book Title: Next Generation Sequencing
Book Subtitle: Methods and Protocols
Editors: Steven R. Head, Phillip Ordoukhanian, Daniel R. Salomon
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7514-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2018
Hardcover ISBN: 978-1-4939-7512-9Published: 10 December 2017
Softcover ISBN: 978-1-4939-8507-4Published: 22 June 2018
eBook ISBN: 978-1-4939-7514-3Published: 09 December 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 266
Number of Illustrations: 10 b/w illustrations, 41 illustrations in colour
Topics: Human Genetics