Editors:
- Includes cutting-edge methods and protocols.
- Provides step-by-step essential for reproducible results.
- Contains key notes and implementation advice from the expert
- Includes supplementary material: sn.pub/extras
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1640)
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Table of contents (20 protocols)
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Front Matter
About this book
This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.
Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.
Editors and Affiliations
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Instituto de Biología Molecular y Celular de Plantas, (Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia), Valencia, Spain
Alberto Carbonell
Bibliographic Information
Book Title: Plant Argonaute Proteins
Book Subtitle: Methods and Protocols
Editors: Alberto Carbonell
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7165-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7164-0Published: 13 June 2017
Softcover ISBN: 978-1-4939-8404-6Published: 12 August 2018
eBook ISBN: 978-1-4939-7165-7Published: 12 June 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 297
Number of Illustrations: 20 b/w illustrations, 27 illustrations in colour
Topics: Plant Sciences