Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1619)
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Table of contents (35 protocols)
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Front Matter
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Updated Blood Processing and Handling Strategies
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Front Matter
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Updated Fractionation Strategies for In-depth Blood Proteome Analysis
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Front Matter
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Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids
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Front Matter
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About this book
Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.
Editors and Affiliations
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Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
David W. Greening, Richard J. Simpson
Bibliographic Information
Book Title: Serum/Plasma Proteomics
Book Subtitle: Methods and Protocols
Editors: David W. Greening, Richard J. Simpson
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7057-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017
Hardcover ISBN: 978-1-4939-7056-8Published: 04 July 2017
Softcover ISBN: 978-1-4939-8380-3Published: 26 August 2018
eBook ISBN: 978-1-4939-7057-5Published: 03 July 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 551
Number of Illustrations: 24 b/w illustrations, 70 illustrations in colour
Topics: Protein Science